Dedysh Svetlana N, Beletsky Alexey V, Ivanova Anastasia A, Danilova Olga V, Begmatov Shahjahon, Kulichevskaya Irina S, Mardanov Andrey V, Ravin Nikolai V
Research Center of Biotechnology of the Russian Academy of Sciences, Winogradsky Institute of Microbiology, 119071 Moscow, Russia.
Research Center of Biotechnology of the Russian Academy of Sciences, Institute of Bioengineering, 119071 Moscow, Russia.
Microorganisms. 2021 Dec 11;9(12):2566. doi: 10.3390/microorganisms9122566.
Methanotrophic verrucomicrobia of the order are known as extremely acidophilic, thermophilic or mesophilic bacteria that inhabit acidic geothermal ecosystems. The occurrence of verrucomicrobial methanotrophs in other types of acidic environments remains an open question. Notably, -affiliated 16S rRNA gene sequences are commonly retrieved from acidic (pH 3.5-5.5) peatlands. In this study, we compared the patterns of verrucomicrobial diversity in four acidic raised bogs and six neutral fens located in European North Russia. -like 16S rRNA gene reads displaying 83-86% similarity to 16S rRNA gene sequences of currently described verrucomicrobial methanotrophs were recovered exclusively from raised bogs. Laboratory incubation of peat samples with 10% methane for 3 weeks resulted in the pronounced increase of a relative abundance of alphaproteobacterial methanotrophs, while no response was detected for -affiliated bacteria. Three metagenome-assembled genomes (MAGs) of peat-inhabiting bacteria were reconstructed and examined for the presence of genes encoding methane monooxygenase enzymes and autotrophic carbon fixation pathways. None of these genomic determinants were detected in assembled MAGs. Metabolic reconstructions predicted a heterotrophic metabolism, with a potential to hydrolyze several plant-derived polysaccharides. As suggested by our analysis, peat-inhabiting representatives of the are acidophilic aerobic heterotrophs, which comprise a sister family of the methanotrophic .
目 的甲烷营养疣微菌是已知的栖息于酸性地热生态系统的极端嗜酸、嗜热或嗜中温细菌。在其他类型的酸性环境中是否存在疣微菌甲烷营养菌仍是一个悬而未决的问题。值得注意的是，通常从酸性（pH 3.5 - 5.5）泥炭地中检索到与目 相关的16S rRNA基因序列。在本研究中，我们比较了位于俄罗斯欧洲部分北部的四个酸性高位沼泽和六个中性沼泽中疣微菌多样性的模式。与目前描述的疣微菌甲烷营养菌的16S rRNA基因序列显示出83 - 86%相似性的类似 16S rRNA基因读数仅从高位沼泽中获得。将泥炭样品与10%甲烷进行实验室培养3周导致α-变形菌纲甲烷营养菌的相对丰度显著增加，而与目 相关的细菌未检测到反应。重建了三个栖息于泥炭地的目 细菌的宏基因组组装基因组（MAGs），并检测了编码甲烷单加氧酶和自养碳固定途径的基因的存在情况。在组装的MAGs中未检测到这些基因组决定因素。代谢重建预测其为异养代谢，具有水解几种植物衍生多糖的潜力。正如我们的分析所表明的，栖息于泥炭地的目 代表是嗜酸需氧异养菌，它们构成了甲烷营养菌目的一个姐妹家族。
