Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, Jagiellonian University Collegium Medicum, 9 Medyczna Street, 30-688, Cracow, Poland.
Faculty of Chemistry, Jagiellonian University, Cracow, Poland.
AAPS PharmSciTech. 2021 Mar 14;22(3):109. doi: 10.1208/s12249-021-01976-1.
Hydrogel wound dressings are highly effective in the therapy of wounds. Yet, most of them do not contain any active ingredient that could accelerate healing. The aim of this study was to prepare hydrophilic active dressings loaded with an anti-inflammatory compound - trans-resveratrol (RSV) of hydrophobic properties. A special attention was paid to select such a technological strategy that could both reduce the risk of irritation at the application site and ensure the homogeneity of the final hydrogel. RSV dissolved in Labrasol was combined with an aqueous sol of poly(vinyl) alcohol (PVA), containing propylene glycol (PG) as a plasticizer. This sol was transformed into a gel under six consecutive cycles of freezing (-80 °C) and thawing (RT). White, uniform and elastic membranes were successfully produced. Their critical features, namely microstructure, mechanical properties, water uptake and RSV release were studied using SEM, DSC, MRI, texture analyser and Franz-diffusion cells. The cryogels made of 8 % of PVA showed optimal tensile strength (0.22 MPa) and elasticity (0.082 MPa). The application of MRI enabled to elucidate mass transport related phenomena in this complex system at the molecular (detection of PG, confinement effects related to pore size) as well as at the macro level (swelling). The controlled release of RSV from membranes was observed for 48 h with mean dissolution time of 18 h and dissolution efficiency of 35 %. All in all, these cryogels could be considered as a promising new active wound dressings.
水凝胶伤口敷料在治疗伤口方面非常有效。然而，它们大多数都不含有任何可以加速愈合的活性成分。本研究的目的是制备亲水性活性敷料，负载具有抗炎作用的化合物-疏水性白藜芦醇（RSV）。特别注意选择一种既能降低应用部位刺激风险，又能确保最终水凝胶均匀性的技术策略。溶解在 Labrasol 中的 RSV 与含有丙二醇（PG）作为增塑剂的聚乙烯醇（PVA）水溶液结合。该溶液在六个连续的冷冻（-80°C）和解冻（RT）循环下转化为凝胶。成功制备了白色、均匀和有弹性的膜。使用 SEM、DSC、MRI、质地分析仪和 Franz 扩散池研究了它们的关键特性，即微观结构、力学性能、吸水率和 RSV 释放。由 8%的 PVA 制成的冷冻凝胶表现出最佳的拉伸强度（0.22 MPa）和弹性（0.082 MPa）。应用 MRI 能够阐明在这个复杂体系中分子水平（检测 PG、与孔径相关的约束效应）和宏观水平（溶胀）上的质量传递相关现象。RSV 从膜中的控释观察了 48 小时，平均溶解时间为 18 小时，溶解效率为 35%。总之，这些冷冻凝胶可以被认为是一种有前途的新型活性伤口敷料。
