Cavalu Simona, Fritea Luminita, Brocks Marcel, Barbaro Katia, Murvai Gelu, Costea Traian Octavian, Antoniac Iulian, Verona Claudio, Romani Martina, Latini Alessandro, Zilli Romano, Rau Julietta V
Faculty of Medicine and Pharmacy, University of Oradea, P-ta 1 Decembrie 10, 410081 Oradea, Romania.
Biomedical Sciences Doctoral School, Faculty of Medicine and Pharmacy, University of Oradea, P-ta 1 Decembrie 10, 410081 Oradea, Romania.
Materials (Basel). 2020 May 1;13(9):2077. doi: 10.3390/ma13092077.
The properties of poly(vinyl alcohol) (PVA)-based composites recommend this material as a good candidate for the replacement of damaged cartilage, subchondral bone, meniscus, humeral joint and other orthopedic applications. The manufacturing process can be manipulated to generate the desired biomechanical properties. However, the main shortcomings of PVA hydrogels are related to poor strength and bioactivity. To overcome this situation, reinforcing elements are added to the PVA matrix. The aim of our work was to develop and characterize a novel composition based on PVA reinforced with Se-doped TiO nanoparticles and natural hydroxyapatite (HA), for possible orthopedic applications. The PVA/Se-doped TiO composites with and without HA were structurally investigated by FTIR and XRD, in order to confirm the incorporation of the inorganic phase in the polymeric structure, and by SEM and XRF, to evidence the ultrastructural details and dispersion of nanoparticles in the PVA matrix. Both the mechanical and structural properties of the composites demonstrated a synergic reinforcing effect of HA and Se-doped TiO nanoparticles. Moreover, the tailorable properties of the composites were proved by the viability and differentiation potential of the bone marrow mesenchymal stem cells (BMMSC) to osteogenic, chondrogenic and adipogenic lineages. The novel hybrid PVA composites show suitable structural, mechanical and biological features to be considered as a promising biomaterial for articular cartilage and subchondral bone repair.
基于聚乙烯醇（PVA）的复合材料的特性使其成为替代受损软骨、软骨下骨、半月板、肱骨关节及其他骨科应用的理想候选材料。其制造工艺可加以调控以产生所需的生物力学性能。然而，PVA水凝胶的主要缺点与强度和生物活性较差有关。为克服这一情况，在PVA基体中添加了增强元素。我们工作的目的是开发并表征一种基于掺硒二氧化钛纳米颗粒和天然羟基磷灰石（HA）增强的新型PVA复合材料，用于可能的骨科应用。通过傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）对含HA和不含HA的PVA/掺硒二氧化钛复合材料进行结构研究，以确认无机相在聚合物结构中的掺入情况，并通过扫描电子显微镜（SEM）和X射线荧光光谱（XRF）来揭示纳米颗粒在PVA基体中的超微结构细节和分散情况。复合材料的力学性能和结构性能均显示出HA和掺硒二氧化钛纳米颗粒的协同增强效应。此外，骨髓间充质干细胞（BMMSC）向成骨、成软骨和脂肪生成谱系的活力和分化潜能证明了复合材料的可定制性能。新型杂化PVA复合材料展现出合适的结构、力学和生物学特性，有望成为用于关节软骨和软骨下骨修复的生物材料。
