Mai Zhimao, Ye Mai, Wang Youshao, Foong Swee Yeok, Wang Lin, Sun Fulin, Cheng Hao
State Key Laboratory of Tropical Oceanography, Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, China.
Front Microbiol. 2021 Dec 7;12:764974. doi: 10.3389/fmicb.2021.764974. eCollection 2021.
In this study, 16S high-throughput and metagenomic sequencing analyses were employed to explore the changes in microbial community and function with the succession of mangroves (, , and ) along the Merbok river estuary in Malaysia. The sediments of the three mangroves harbored their own unique dominant microbial taxa, whereas exhibited the highest microbial diversity. In general, Gammaproteobacteria, Actinobacteria, Alphaproteobacteria, Deltaproteobacteria, and Anaerolineae were the dominant microbial classes, but their abundances varied significantly among the three mangroves. Principal coordinates and redundancy analyses revealed that the specificity of the microbial community was highly correlated with mangrove populations and environmental factors. The results further showed that exhibited the highest carbon-related metabolism, coinciding with the highest organic carbon and microbial diversity. In addition, specific microbial taxa, such as Desulfobacterales and Rhizobiales, contributed the highest functional activities related to carbon metabolism, prokaryote carbon fixation, and methane metabolism. The present results provide a comprehensive understanding of the adaptations and functions of microbes in relation to environmental transition and mangrove succession in intertidal regions. High microbial diversity and carbon metabolism in might in turn facilitate and maintain the formation of climax mangroves in the middle region of the Merbok river estuary.
在本研究中，采用16S高通量测序和宏基因组测序分析方法，探究马来西亚默博克河口沿岸红树林（、和）演替过程中微生物群落和功能的变化。三种红树林的沉积物中都有各自独特的优势微生物类群，其中的微生物多样性最高。总体而言，γ-变形菌纲、放线菌纲、α-变形菌纲、δ-变形菌纲和厌氧绳菌纲是主要的微生物类群，但它们在三种红树林中的丰度差异显著。主坐标分析和冗余分析表明，微生物群落的特异性与红树林种群和环境因素高度相关。结果还表明，的碳相关代谢最高，这与最高的有机碳和微生物多样性相一致。此外，特定的微生物类群，如脱硫杆菌目和根瘤菌目，在与碳代谢、原核生物碳固定和甲烷代谢相关的功能活动中贡献最大。目前的结果为深入了解潮间带环境转变和红树林演替过程中微生物的适应性和功能提供了全面认识。中高微生物多样性和碳代谢可能反过来促进和维持默博克河口中部地区高潮红树林的形成。
