甲硫氨酸转运RNA与大肠杆菌23S核糖体RNA的特异性结合。
Specific binding of tRNAMet to 23S rRNA of Escherichia coli.
作者信息
Dahlberg J E, Kintner C, Lund E
出版信息
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1071-5. doi: 10.1073/pnas.75.3.1071.
tRNAMetf binds to 23S rRNA of Escherichia coli, forming a complex with a melting temperature of 75 degrees (in 0.6 M NaCl). The regions within the RNAs that bind to each other have been isolated and their nucleotide sequences have been determined. The interacting region in tRNAMetf is 17 nucleotides long, extending from G5 in the acceptor stem to D21 (D = 5.6-dihydrouridine) in the D loop. The sequence in 23S rRNA is complementary to that sequence except for an extra Up in the middle and allowing a Gp.D base pair. We propose that association of these two sequences may play a role in initiation of protein synthesis by tRNAMetf. In addition, part of this sequence in 23S rRNA may also stabilize tRNA binding to the ribosome during elongation of nascent polypeptides.
甲硫氨酸起始转运核糖核酸（tRNAMetf）与大肠杆菌的23S核糖体核糖核酸（rRNA）结合，形成一种解链温度为75摄氏度（在0.6M氯化钠中）的复合物。核糖核酸中相互结合的区域已被分离出来，其核苷酸序列也已确定。tRNAMetf中的相互作用区域长度为17个核苷酸，从受体茎中的G5延伸至D环中的D21（D = 5,6 - 二氢尿苷）。23S rRNA中的序列与该序列互补，只是中间有一个额外的尿苷一磷酸（Up），并允许形成一个鸟苷一磷酸.二氢尿苷（Gp.D）碱基对。我们认为这两个序列的结合可能在tRNAMetf启动蛋白质合成过程中发挥作用。此外，23S rRNA中该序列的一部分在新生多肽延伸过程中也可能稳定转运核糖核酸与核糖体的结合。
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