核糖体RNA的精细结构。IV. rDNA中内含子侧翼序列的异常进化保守性。
Fine structure of ribosomal RNA. IV. Extraordinary evolutionary conservation in sequences that flank introns in rDNA.
作者信息
Gourse R L, Gerbi S A
出版信息
Nucleic Acids Res. 1980 Aug 25;8(16):3623-37. doi: 10.1093/nar/8.16.3623.
By hybridization and DNA sequencing, we have defined a specific region in Xenopus rDNA that is extremely conserved between Tetrahymena, a protozoan, and Xenopus, a vertebrate. This highly conserved region is found at the site where an intron has been shown to interrupt Tetrahymena rDNA [1,2], although we have not detected introns in genomic or cloned Xenopus rDNA. We have noted that the sequences corresponding to nuclear rDNA interon-flanking regions show an intriguing complementarity to tRNAiMet. This suggests possible models for tRNA-rRNA interactions in protein synthesis and/or rRNA splicing.
通过杂交和DNA测序，我们确定了非洲爪蟾核糖体DNA（rDNA）中的一个特定区域，该区域在原生动物四膜虫和脊椎动物非洲爪蟾之间极为保守。尽管我们在基因组或克隆的非洲爪蟾rDNA中未检测到内含子，但这个高度保守的区域位于已显示会中断四膜虫rDNA的内含子所在位置[1,2]。我们注意到，与核糖体DNA内含子侧翼区域相对应的序列与甲硫氨酸起始tRNA（tRNAiMet）呈现出有趣的互补性。这为蛋白质合成和/或核糖体RNA剪接中的tRNA-rRNA相互作用提供了可能的模型。
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