Liebert U G, ter Meulen V
J Gen Virol. 1987 Jun;68 ( Pt 6):1715-22. doi: 10.1099/0022-1317-68-6-1715.
Lewis and Brown Norway (BN) rats which are susceptible or resistant to autoimmune reactions against brain antigen, respectively, were inoculated intracerebrally with a neurotropic measles virus. Suckling rats died from a rapidly fatal acute encephalopathy (AE). With increasing age Lewis rats developed a subacute measles encephalomyelitis (SAME) whereas BN rats showed a clinically silent encephalitis (CSE). Infectious virus could occasionally be recovered from SAME animals using cocultivation techniques but not from BN rats with CSE. With monoclonal antibodies against measles virus, viral proteins were localized in brain tissue. Nucleocapsid and phosphoprotein were detected in infected brain cells of all animals with AE, SAME and CSE, whereas measles virus haemagglutinin, fusion and matrix proteins were either reduced or absent, suggesting a restricted synthesis of measles virus envelope proteins. These data suggest that the different diseases of the two rat strains are related to the immunogenetic background rather than to the replication of measles virus in the central nervous system. This animal model provides the opportunity to investigate further the events occurring during establishment of measles virus persistence in the brain, and the genetic control of associated immunological and immunopathological reactions.
分别对脑抗原有自身免疫反应易感性或抗性的刘易斯大鼠和布朗挪威（BN）大鼠，经脑内接种嗜神经性麻疹病毒。乳鼠死于迅速致命的急性脑病（AE）。随着年龄增长，刘易斯大鼠发展为亚急性麻疹脑脊髓炎（SAME），而BN大鼠表现为临床无症状性脑炎（CSE）。使用共培养技术偶尔能从患有SAME的动物中分离出感染性病毒，但从患有CSE的BN大鼠中则无法分离出。用抗麻疹病毒单克隆抗体将病毒蛋白定位在脑组织中。在所有患有AE、SAME和CSE的动物的感染脑细胞中均检测到核衣壳蛋白和磷蛋白，而麻疹病毒血凝素、融合蛋白和基质蛋白则减少或缺失，这表明麻疹病毒包膜蛋白的合成受到限制。这些数据表明，两种大鼠品系的不同疾病与免疫遗传背景有关，而非与麻疹病毒在中枢神经系统中的复制有关。该动物模型为进一步研究麻疹病毒在脑中持续存在期间发生的事件以及相关免疫和免疫病理反应的遗传控制提供了机会。
