牛蒡水溶性多糖的结构表征及其对结肠炎小鼠的作用。
Structural characterization of water-soluble polysaccharide from Arctium lappa and its effects on colitis mice.
机构信息
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China.
College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, Jiangsu, China.
出版信息
Carbohydr Polym. 2019 Jun 1;213:89-99. doi: 10.1016/j.carbpol.2019.02.090. Epub 2019 Feb 27.
In this study, water-soluble polysaccharide from Arctium lappa was extracted, isolated and purified to be a fraction (ALP-1). Characterization of structure revealed that ALP-1 was a kind of fructan with a molecular weight of 5.12 × 10 Da. ALP-1 was composed of (2→ 1)-β-d-fructofuranose backbone linked to a terminal of (2 → 1)-α-d-glucopyranose at the non-reducing end and a (2 → 6)-β-d-fructofuranose branching. DSS-induced colitis mice were used to determine the inhibitory effects of ALP-1 on gut inflammation. Results indicated that ALP-1 could significantly ameliorate the dysregulation of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) and anti-inflammatory cytokine (IL-10) caused by colitis. Besides, as compared with model group, the abundance of Firmicutes, Ruminococcaceae, Lachnospiraceae and Lactobacillus were significantly increased with ALP-1 treatment. And ALP-1 could significantly inhibit the levels of Proteobacteria, Alcaligenaceae, Staphylococcusand and Bacteroidetes. Therefore, ALP-1 may be effective in protecting mice from DSS-induced colitis.
在这项研究中，从牛蒡中提取、分离和纯化了水溶性多糖，得到了一个馏分（ALP-1）。结构表征表明，ALP-1 是一种果聚糖，分子量为 5.12×10 Da。ALP-1 由（2→1）-β-d-吡喃果糖苷骨架与非还原端的（2→1）-α-d-吡喃葡萄糖连接，末端连接一个（2→6）-β-d-吡喃果糖支链。用 DSS 诱导结肠炎小鼠来确定 ALP-1 对肠道炎症的抑制作用。结果表明，ALP-1 可显著改善结肠炎引起的促炎细胞因子（IL-1β、IL-6 和 TNF-α）和抗炎细胞因子（IL-10）的失调。此外，与模型组相比，ALP-1 处理可显著增加厚壁菌门、瘤胃球菌科、毛螺菌科和乳杆菌的丰度。并且，ALP-1 可显著抑制变形菌门、产碱杆菌科、葡萄球菌和拟杆菌门的水平。因此，ALP-1 可能对保护小鼠免受 DSS 诱导的结肠炎有效。
Zotero 插件