Key Laboratory of Marine Geology and Environment, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, PR China.
Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005152.
A Gram-stain-negative, motile, facultative anaerobic and rod-shaped bacterium, designated strain NR704-98, was isolated from marine sediment of the northern South China Sea. Cells were positive for oxidase and catalase activity. Growth was observed at 4-30 °C (optimum 20-25 °C), at pH 6-9 (pH 7) and with 0.5-7 % NaCl (2 %). The 16S rRNA gene-based phylogenetic analysis revealed that the nearest phylogenetic neighbours of strain NR704-98 were MS32 (97.9 %), 281 (97.1 %), HAW-EB3 (96.8 %) and HAW-EB2 (96.7 %). Based on the results of phylogenomic analysis, the average nucleotide identity and the digital DNA-DNA hybridization values between strain NR704-98 and the previously mentioned type strains of species of the genus were in the range of 74.9-93.1 % and 20.6-51.4 %, respectively. The respiratory quinones were Q-7 and Q-8. The predominant fatty acids (>10 %) of strain NR704-98 were C, summed feature 3 (C 7 and/or C 6) and iso-C. Phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminophospholipids and five unidentified lipids were detected in strain NR704-98. Based on the phylogenetic and phenotypic characteristics, strain NR704-98 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NR704-98 (=KCTC 82799=MCCC 1K06091).
一株革兰氏阴性、运动性、兼性厌氧的杆状细菌，被命名为 NR704-98 菌株，从南海北部海洋沉积物中分离得到。细胞氧化酶和过氧化氢酶活性均为阳性。在 4-30°C（最佳温度 20-25°C）、pH6-9（pH7）和 0.5-7%NaCl（2%）的条件下可以观察到生长。基于 16S rRNA 基因的系统发育分析表明，NR704-98 菌株的最近的系统发育同源菌株是 MS32（97.9%）、281（97.1%）、HAW-EB3（96.8%）和 HAW-EB2（96.7%）。基于基因组分析的结果，NR704-98 菌株与上述属种的模式菌株的平均核苷酸同一性和数字 DNA-DNA 杂交值分别在 74.9-93.1%和 20.6-51.4%的范围内。呼吸醌为 Q-7 和 Q-8。NR704-98 菌株的主要脂肪酸（>10%）为 C、总和特征 3（C 7 和/或 C 6）和 iso-C。NR704-98 菌株中检测到磷脂酰乙醇胺、磷脂酰甘油、两种未鉴定的氨基磷脂和五种未鉴定的脂类。根据系统发育和表型特征，NR704-98 菌株被认为代表了一个新的属种，建议将其命名为 sp. nov.。其模式菌株为 NR704-98（=KCTC 82799=MCCC 1K06091）。
