Bahler D W, Frelinger J G, Harwell L W, Lord E M
Proc Natl Acad Sci U S A. 1987 Jul;84(13):4562-6. doi: 10.1073/pnas.84.13.4562.
Cultured cells of the murine lung carcinoma called line 1 express very low levels of H-2 class I antigens and are resistant to lysis mediated by alloreactive T cells. In order to investigate how the expression of class I antigens affects the in vivo growth of this spontaneous tumor, H-2Dp genes were transferred into line 1 cells. Cloned transfectants that displayed H-2Dp surface antigens were identified using flow cytometry. The transfected H-2Dp antigens appeared normal by two-dimensional gel electrophoresis and could also function as excellent targets for T-cell-mediated lysis in vitro. Marked differences in tumorigenicity (defined as tumor growth in immunologically competent hosts) were observed between the Dp transfected cells and untransfected or control transfected line 1 cells in syngeneic mice only if the animals had previously received injections of irradiated Dp transfectants. Expression of Dp antigens did not appreciably affect the growth of line 1 tumors in immunologically naive syngeneic mice or necessarily cause rejection in allogeneic mice. Our in vivo results show that increased expression of class I antigens can reduce the growth of tumors like line 1 that lack all class I antigens. Our results also suggest that increasing class I antigens alone on some spontaneous tumors deficient in expression will not by itself be sufficient for tumor rejection.
一种名为1号线的小鼠肺癌培养细胞表达的H-2 I类抗原水平极低，并且对同种反应性T细胞介导的裂解具有抗性。为了研究I类抗原的表达如何影响这种自发性肿瘤的体内生长，将H-2Dp基因转入1号线细胞。使用流式细胞术鉴定出显示H-2Dp表面抗原的克隆转染子。通过二维凝胶电泳，转染的H-2Dp抗原看起来正常，并且在体外也可以作为T细胞介导的裂解的良好靶标。仅当动物先前接受过辐照的Dp转染子注射时，在同基因小鼠中，Dp转染细胞与未转染或对照转染的1号线细胞之间才观察到致瘤性（定义为在免疫活性宿主中的肿瘤生长）存在明显差异。Dp抗原的表达并未明显影响免疫未致敏的同基因小鼠中1号线肿瘤的生长，也不一定会导致异基因小鼠中的肿瘤排斥。我们的体内结果表明，I类抗原表达的增加可以减少像1号线这样缺乏所有I类抗原的肿瘤的生长。我们的结果还表明，仅在一些表达缺陷的自发性肿瘤上增加I类抗原本身不足以实现肿瘤排斥。
