Neglo David, Tettey Clement Okraku, Essuman Edward Ken, Amenu Justice Dziedzorm, Mills-Robertson Felix Charles, Sedohia Daniel, Boakye Adjoa Agyemang, Abaye Daniel A
Department of Basic Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, PMB 31, Ho, Volta Region, Ghana.
Department of Biochemistry and Biotechnology, Kwame Nkrumah University of Science and Technology, University Post, Kumasi, AR, Ghana.
Evid Based Complement Alternat Med. 2021 Dec 20;2021:9342110. doi: 10.1155/2021/9342110. eCollection 2021.
The study investigated the influence of extracts on the action of selected antibiotics against biofilm-forming MRSA. The various parts of the plant were processed into powder and extracted with ethanol or hot water and then screened for the presence of phytochemicals. The modulatory effect of the extract was also tested on some antibiotics against Methicillin-resistant (MRSA). The findings from this study revealed that the various parts of the extract (ethanolic and aqueous) contained different proportions of secondary metabolites. Varied antimicrobial activities were observed when the extract of the was exposed to MRSA strain at a concentration of 100 mg/mL. The stem recorded the highest (17.00 and 18.00 mm) inhibitory activity against MRSA for both the aqueous and the ethanolic extract, respectively, and this was not different from the control, tetracycline. Again, the results on the modulatory action indicated that out of the 10 extracts of , 4 of them antagonized the activity of ampicillin against the tested MRSA by a factor of 0.5 folds and the rest potentiated the drug within 1-4 folds, respectively. On the other hand, the various test extracts significantly potentiated the efficacy of streptomycin and tetracycline against the MRSA by a range of 1-32 folds with the aqueous root extract recording the highest synergistic effect and ethanol seed extract with the least effect. The findings of this study support the antibacterial activities of the plant parts.
该研究调查了提取物对所选抗生素抗生物膜形成的耐甲氧西林金黄色葡萄球菌（MRSA）作用的影响。将植物的各个部分加工成粉末，用乙醇或热水提取，然后筛选植物化学物质的存在。还测试了该提取物对一些抗耐甲氧西林金黄色葡萄球菌（MRSA）抗生素的调节作用。这项研究的结果表明，该提取物的各个部分（乙醇提取物和水提取物）含有不同比例的次生代谢物。当该提取物以100 mg/mL的浓度暴露于MRSA菌株时，观察到了不同的抗菌活性。茎部对MRSA的抑制活性最高（水提取物和乙醇提取物分别为17.00和18.00 mm），这与对照四环素没有差异。此外，调节作用的结果表明，在该植物的10种提取物中，有4种将氨苄西林对测试的MRSA的活性拮抗了0.5倍，其余的则分别将药物增效了1至4倍。另一方面，各种测试提取物使链霉素和四环素对MRSA的疗效显著增强了1至32倍，其中水根提取物的协同效应最高，乙醇种子提取物的效应最小。这项研究的结果支持了该植物各部分的抗菌活性。
