Gheata Adrian, Spada Alessandra, Wittwer Manon, Dhouib Ameni, Molina Emilie, Mugnier Yannick, Gerber-Lemaire Sandrine
Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, Group for Functionalized Biomaterials, 1015 Lausanne, Switzerland.
Département de Chimie, École Normale Supérieure, PSL University, 75005 Paris, France.
Nanomaterials (Basel). 2023 Jan 28;13(3):522. doi: 10.3390/nano13030522.
Inorganic nanoparticles (NPs) have emerged as promising tools in biomedical applications, owing to their inherent physicochemical properties and their ease of functionalization. In all potential applications, the surface functionalization strategy is a key step to ensure that NPs are able to overcome the barriers encountered in physiological media, while introducing specific reactive moieties to enable post-functionalization. Silanization appears as a versatile NP-coating strategy, due to the biocompatibility and stability of silica, thus justifying the need for robust and well controlled silanization protocols. Herein, we describe a procedure for the silica coating of harmonic metal oxide NPs (LiNbO, LNO) using a water-in-oil microemulsion (W/O ME) approach. Through optimized ME conditions, the silanization of LNO NPs was achieved by the condensation of silica precursors (TEOS, APTES derivatives) on the oxide surface, resulting in the formation of coated NPs displaying carboxyl () or azide () reactive moieties. NPs were further conjugated to an unnatural azido-containing small peptide to obtain silica-coated LNO NPs (), displaying both azide and carboxyl moieties, which are well suited for biomedical applications due to the orthogonality of their surface functional groups, their colloidal stability in aqueous medium, and their anti-fouling properties.
无机纳米粒子（NPs）因其固有的物理化学性质和易于功能化的特点，已成为生物医学应用中有前景的工具。在所有潜在应用中，表面功能化策略是确保纳米粒子能够克服生理介质中遇到的障碍，同时引入特定反应基团以实现后功能化的关键步骤。由于二氧化硅的生物相容性和稳定性，硅烷化似乎是一种通用的纳米粒子涂层策略，因此有必要制定稳健且可控的硅烷化方案。在此，我们描述了一种使用油包水微乳液（W/O ME）方法对谐波金属氧化物纳米粒子（LiNbO，LNO）进行二氧化硅涂层的程序。通过优化微乳液条件，LNO纳米粒子的硅烷化是通过二氧化硅前体（TEOS、APTES衍生物）在氧化物表面的缩合实现的，从而形成了带有羧基（）或叠氮基（）反应基团的包覆纳米粒子。纳米粒子进一步与一种含非天然叠氮基的小肽共轭，以获得同时具有叠氮基和羧基部分的二氧化硅包覆的LNO纳米粒子（），由于其表面官能团的正交性、在水性介质中的胶体稳定性和抗污性能，它们非常适合生物医学应用。
