使用GraphPad Prism从糖组学微阵列数据计算半数最大抑制浓度（IC）值。
Calculating Half Maximal Inhibitory Concentration (IC) Values from Glycomics Microarray Data Using GraphPad Prism.
作者信息
Le Berre Marie, Gerlach Jared Q, Dziembała Iwona, Kilcoyne Michelle
机构信息
Carbohydrate Signalling Group, Discipline of Microbiology, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
Advanced Glycoscience Research Cluster, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland.
出版信息
Methods Mol Biol. 2022;2460:89-111. doi: 10.1007/978-1-0716-2148-6_6.
Half maximal inhibitory concentration (IC) is a measurement often used to compare the efficiency of various carbohydrates and their derivatives for inhibition of lectin binding to particular ligands. IC values can be calculated using experimental data from various platforms including enzyme-linked immunosorbent assay- (ELISA-)type microtiter plate assays, isothermal titration calorimetry (ITC), or glycan microarrays. In this chapter, we describe methods to fluorescently label a lectin, to carry out a lectin binding inhibition experiment on glycan microarrays, and to calculate the IC value of a binding inhibitory molecule using GraphPad Prism software. In the example used to illustrate the method in this chapter, IC calculation is demonstrated for inhibition of Maackia amurensis agglutinin (MAA) binding to 3'sialyl-N-acetyllactosamine (3SLN) using free lactose.
半数最大抑制浓度（IC）是一种常用于比较各种碳水化合物及其衍生物抑制凝集素与特定配体结合效率的测量方法。IC值可使用来自各种平台的实验数据进行计算，包括酶联免疫吸附测定（ELISA）型微孔板测定、等温滴定量热法（ITC）或聚糖微阵列。在本章中，我们描述了对凝集素进行荧光标记、在聚糖微阵列上进行凝集素结合抑制实验以及使用GraphPad Prism软件计算结合抑制分子IC值的方法。在本章用于说明该方法的示例中，展示了使用游离乳糖抑制黑穗醋栗凝集素（MAA）与3'-唾液酸-N-乙酰乳糖胺（3SLN）结合的IC计算。
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