Gutierrez C, Martin M J, Brown K A
Complement. 1987;4(2):99-109. doi: 10.1159/000463013.
A study was made of the complement activation (CA) capacity of human lymphocytes from lymphoid organs. Whereas thymocytes did not show complement membrane fluorescence (CMF) following incubation in normal homologous serum (NHS), a mean of 18 and 34%, respectively, of tonsil and spleen cells were positive for antihuman C3 fluorescent serum. Isolated T cells did not show CA capacity, while 62% of a purified tonsil B population possessed this capacity. The CMF was abolished by adding EDTA and when the incubation was performed in C1q and factor-D-depleted serum. Addition of EGTA, supplemented with MgCl2 to NHS failed to abolish the fluorescence, indicating that the alternative pathway was involved in the phenomenon. Removal of sialic acid with neuraminidase increased the percentage of cells showing CMF. Since sialic acid significantly contributes to the extent of net negative surface charge, we have studied whether this parameter influences the CA capacity. Tonsil B cells were separated in fractions with different electrophoretic mobility by preparative cell electrophoresis, and similar percentages of CMF-positive cells were found in all fractions. Therefore, it is likely that the capacity to activate complement is unrelated to the membrane electrical charge.
对来自淋巴器官的人淋巴细胞的补体激活（CA）能力进行了研究。在正常同源血清（NHS）中孵育后，胸腺细胞未显示补体膜荧光（CMF），而扁桃体细胞和脾细胞分别平均有18%和34%对抗人C3荧光血清呈阳性。分离的T细胞未显示CA能力，而纯化的扁桃体B细胞群体中有62%具有此能力。加入EDTA以及在C1q和D因子缺失的血清中进行孵育时，CMF消失。向NHS中添加补充有MgCl2的EGTA未能消除荧光，表明替代途径参与了该现象。用神经氨酸酶去除唾液酸增加了显示CMF的细胞百分比。由于唾液酸对净负表面电荷的程度有显著贡献，我们研究了该参数是否影响CA能力。通过制备性细胞电泳将扁桃体B细胞分离成具有不同电泳迁移率的组分，在所有组分中发现CMF阳性细胞的百分比相似。因此，激活补体的能力可能与膜电荷无关。
