Government College of Arts and Science, Kurumbalur, Perambalur, (Formerly, Bharathidasan University Constituent College, Perambalur), Kurumbalur, Tamil Nadu, 621212, India.
DBT-National Centre for Cell Science, Pune, 411007, India.
Arch Microbiol. 2022 Jan 3;204(1):110. doi: 10.1007/s00203-021-02728-2.
Bacterial diversity of four thermally different hot springs of Ratnagiri district, Maharashtra, India, was investigated using culture-dependent and culture-independent approaches. A total of 144 bacterial cultures were isolated and identified using MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry) and 16S rRNA gene sequencing. Culture-independent analysis by Ion Torrent sequencing targeting the V3 region of the 16S rRNA gene revealed the predominance of Firmicutes across all the hot springs, followed by Chloroflexi, Bacteroidetes, Cyanobacteria, Proteobacteria, Armatimonadetes, Actinobacteria, Nitrospirae, Acidobacteria, and Deinococcus-Thermus, with subtle differences in their abundance. At the lower taxonomic rank of genus, we noted the prevalence of Acinetobacter followed by Clostridium, Planomicrobium, Bacillus, Streptomyces, and Leptolyngbya. Metagenomics imputation using in silico approach revealed divergence in the metabolic capabilities of bacterial communities along the thermal gradient of host springs, with site TS (63 °C) featuring the abundant functional gene families.
采用培养依赖和培养独立的方法研究了印度马哈拉施特拉邦拉特纳吉里地区四个温度不同的温泉的细菌多样性。使用 MALDI-TOF MS（基质辅助激光解吸电离飞行时间质谱）和 16S rRNA 基因测序对总共 144 个细菌培养物进行了分离和鉴定。针对 16S rRNA 基因 V3 区的 Ion Torrent 测序的非培养分析显示，所有温泉中厚壁菌门都占优势，其次是绿弯菌门、拟杆菌门、蓝藻门、变形菌门、装甲门、放线菌门、硝化螺旋菌门、酸杆菌门和 Deinococcus-Thermus，其丰度存在细微差异。在属的较低分类等级上，我们注意到以下属的流行：不动杆菌属，其次是梭菌属、Planomicrobium 属、芽孢杆菌属、链霉菌属和 Leptolyngbya 属。使用计算机模拟方法进行宏基因组推测表明，随着宿主温泉温度梯度的变化，细菌群落的代谢能力存在差异，TS 点（63°C）具有丰富的功能基因家族。
