Dingle J T, Page Thomas D P, Hazleman B
Int J Tissue React. 1987;9(4):349-54.
The injection of partially purified porcine synovial catabolin/IL-1 alpha intra-articularly in rabbits resulted in a 50% loss of glycosaminoglycan (GAG) after 3 days. An increase in the synovial fluid content of GAG was found, and 35SO4 incorporation into proteoglycan was inhibited. Measurements were also made of the GAG loss from articular cartilage into the synovial fluid in human rheumatoid (RA) and osteoarthritic (OA) patients. Very high levels of GAG content in the synovial fluid was found, and calculations were made of the half-life of the cartilage proteoglycan during the active phases of the disease. Estimation of the synovial fluid GAG is believed to be a simple and quantitative method for monitoring the effectiveness of cartilage-"sparing" anti-arthritic drugs.
给兔子关节内注射部分纯化的猪滑膜分解代谢素/白细胞介素-1α，3天后糖胺聚糖（GAG）损失50%。发现滑液中GAG含量增加，且35SO4掺入蛋白聚糖受到抑制。还对人类类风湿性关节炎（RA）和骨关节炎（OA）患者关节软骨中GAG向滑液的流失进行了测量。发现滑液中GAG含量非常高，并计算了疾病活动期软骨蛋白聚糖的半衰期。滑膜液GAG的评估被认为是监测软骨保护型抗关节炎药物疗效的一种简单定量方法。
