Zhejiang University-University of Edinburgh (ZJU-UoE) Institute, Zhejiang University, Haining, Zhejiang 310027, P.R. China.
The Second Affiliated Hospital Zhejiang University (SAHZU), School of Medicine, Hangzhou, Zhejiang 310058, P.R. China.
ACS Infect Dis. 2022 Jan 14;8(1):59-65. doi: 10.1021/acsinfecdis.1c00117. Epub 2022 Jan 3.
Non-antibiotic alternative treatments to combat the increasing number of infections caused by multidrug resistant bacteria are urgently needed. In recent years, bacteriophages have reemerged to potentially replace or complement the role of antibiotics, as bacterial viruses have the ability to inactivate pathogens. This study aimed to evaluate the synergy of phage-antibiotic combinations. A isolate was used in this study together with the phage MRM57. Eight different antibiotics with different mechanisms of action were used in combination with the phage to study the impact of the combination treatment on the minimal inhibitory concentrations. We found that antibiotic concentration dependent synergism exists, albeit at different extents, with very low numbers of phages. This demonstrates the use of phages as an adjuvant with a sublethal concentration of antibiotics as an effective therapeutic strategy.
急需非抗生素替代疗法来应对由多药耐药菌引起的感染数量不断增加的问题。近年来，噬菌体重新出现，可能会替代或补充抗生素的作用，因为细菌病毒具有使病原体失活的能力。本研究旨在评估噬菌体-抗生素联合治疗的协同作用。本研究中使用了一株 分离株和噬菌体 MRM57。使用了 8 种不同作用机制的抗生素与噬菌体联合使用，以研究联合治疗对最小抑菌浓度的影响。我们发现，尽管程度不同，但在非常低数量的噬菌体存在下，抗生素浓度依赖性协同作用确实存在。这表明，将噬菌体作为亚致死浓度抗生素的佐剂使用是一种有效的治疗策略。
