Doern G V, Daum G S, Tubert T A
J Clin Microbiol. 1987 Aug;25(8):1453-5. doi: 10.1128/jcm.25.8.1453-1455.1987.
The activity of chloramphenicol against 100 different strains of Haemophilus influenzae was assessed by a macrotube broth dilution technique and by a standardized disk diffusion method using both enriched chocolate agar (CHOC) and Mueller-Hinton agar containing 1.0% hemoglobin and 1.0% IsoVitaleX (BBL Microbiology Systems, Cockeysville, Md.) supplement (CHOC-MHA). Filter disks containing 30 micrograms of chloramphenicol were used with the disk diffusion procedure. The following zone diameter interpretive criteria were defined: CHOC-MHA, less than or equal to 25 mm = resistant [corrected] and greater than or equal to 26 mm = susceptible [corrected]; CHOC, less than or equal to 28 mm = resistant [corrected] and greater than or equal to 29 mm = susceptible [corrected]. all of the H. influenzae strains examined were also characterized by using two rapid assays for chloramphenicol acetyltransferase (CAT) activity: a 1-h tube method (t-CAT) and a 30-min procedure which used commercially available reagent-impregnated disks (d-CAT). The t-CAT procedure was found to be significantly more accurate than the d-CAT procedure as a means for demonstrating production of CAT.
采用大管肉汤稀释技术以及使用富含巧克力的琼脂（CHOC）和含1.0%血红蛋白与1.0% IsoVitaleX（BBL微生物系统公司，马里兰州科基斯维尔）添加剂的穆勒-欣顿琼脂（CHOC-MHA）的标准化纸片扩散法，评估氯霉素对100株不同流感嗜血杆菌菌株的活性。纸片扩散法使用含30微克氯霉素的滤纸片。定义了以下抑菌圈直径解释标准：CHOC-MHA，小于或等于25毫米=耐药[校正后]，大于或等于26毫米=敏感[校正后]；CHOC，小于或等于28毫米=耐药[校正后]，大于或等于29毫米=敏感[校正后]。还通过两种氯霉素乙酰转移酶（CAT）活性快速检测方法对所有检测的流感嗜血杆菌菌株进行了特征鉴定：1小时试管法（t-CAT）和使用市售试剂浸渍纸片的30分钟方法（d-CAT）。作为证明CAT产生的方法，发现t-CAT方法比d-CAT方法显著更准确。
