Marklund S L, Repo H, Koskimies S
Acta Pathol Microbiol Immunol Scand C. 1987 Jun;95(3):107-11. doi: 10.1111/j.1699-0463.1987.tb00016.x.
We studied the activity of superoxide dismutase (SOD) isoenzymes (CuZnSOD, cyanide-resistant MnSOD and extracellular SOD), the concentration of plasma selenium (P-Se), and the activities of CuZnSOD and glutathione peroxidase (GSHPx) in erythrocytes of 10 HLA-B27-positive and 10 HLA-B27-negative subjects whose sera had shown high and low chemokinetic activity, respectively, on migration of polymorphonuclear leukocytes (PMN) in vitro. No significant difference was found between the groups, suggesting that the enhanced chemokinetic activity of HLA-B27-positive sera does not derive from an aberration in serum anti-oxidant potentials. The results were also analysed on the basis of HLA-DR specificities; HLA-DR4-positive plasma samples had significantly lower levels of Se than HLA-DR4-negative ones. We therefore carried out a second series of experiments with sera of 37 subjects; the levels of P-Se in HLA-DR4-positive and -negative groups were much the same, as were the activities of GSHPx. The data suggest that there is no gross aberration in P-Se concentration in HLA-DR4-positive subjects.
我们研究了10名HLA - B27阳性和10名HLA - B27阴性受试者红细胞中超氧化物歧化酶（SOD）同工酶（铜锌超氧化物歧化酶、抗氰锰超氧化物歧化酶和细胞外超氧化物歧化酶）的活性、血浆硒（P - Se）浓度以及铜锌超氧化物歧化酶和谷胱甘肽过氧化物酶（GSHPx）的活性。这些受试者的血清在体外多形核白细胞（PMN）迁移中分别表现出高和低的趋化活性。两组之间未发现显著差异，这表明HLA - B27阳性血清增强的趋化活性并非源于血清抗氧化潜能的异常。结果还根据HLA - DR特异性进行了分析；HLA - DR4阳性血浆样本的硒水平显著低于HLA - DR4阴性样本。因此，我们用37名受试者的血清进行了第二系列实验；HLA - DR4阳性和阴性组的P - Se水平大致相同，GSHPx的活性也是如此。数据表明HLA - DR4阳性受试者的P - Se浓度没有明显异常。
