LAQV-REQUIMTE, Campus da Caparica, Monte da Caparica, 2825-149, Caparica, Portugal.
LAQV-REQUIMTE, Campus da Caparica, Monte da Caparica, 2825-149, Caparica, Portugal.
Cryobiology. 2021 Aug;101:95-104. doi: 10.1016/j.cryobiol.2021.05.002. Epub 2021 May 8.
This work aimed at evaluating the potential of using natural deep eutectic systems (NADES) as cryoprotectant agents (CPAs). Several combinations between natural primary metabolites that have been identified in animals that live in extreme cold climates were prepared. All systems showed very little cytoxicity towards L929 cells at concentrations high as 1-2 M. Moreover, this cell line was highly tolerant to 10% (w/v) of NADES when compared to MeSO. To test NADES as CPAs, two cell lines were used, L929 and HacaT cells. After freeze/thawing cycle, it was possible to observe that for L929 cells, NADES presented similar behaviour to MeSO. For Hacat cell line a significant improvement on post-thawing recovery was observed. Moreover, the results presented herein showed that NADES do not need to be removed from the freezing media after thawing the cells, which is a great advantage of these materials. Additionally, we have shown that NADES can act as CPA when cells are frozen at -20 °C. In overall, the results demonstrate the high potential of NADES to be used in cryobiology as alternative CPAs.
本研究旨在评估天然深共晶溶剂（NADES）作为冷冻保护剂（CPA）的潜力。制备了几种在生活在极寒环境中的动物中鉴定出的天然初级代谢物之间的组合。所有系统在高达 1-2 M 的浓度下对 L929 细胞的细胞毒性都非常低。此外，与 MeSO 相比，该细胞系对 10%（w/v）的 NADES 具有很高的耐受性。为了测试 NADES 作为 CPA，使用了两种细胞系，L929 和 HacaT 细胞。在冻融循环后，观察到对于 L929 细胞，NADES 的行为与 MeSO 相似。对于 Hacat 细胞系，观察到解冻后细胞恢复活力有显著提高。此外，本文的结果表明，NADES 不需要在细胞解冻后从冷冻培养基中去除，这是这些材料的一个很大的优势。此外，我们已经表明，当细胞在-20°C 下冷冻时，NADES 可以作为 CPA 发挥作用。总的来说，这些结果表明 NADES 作为替代 CPA 在 cryobiology 中具有很高的应用潜力。
