College of Pharmaceutical Science, Dali University, Dali, China.
Key Laboratory of Yunnan Provincial Higher Education Institutions for Development of Yunnan Daodi Medicinal Materials Resources, Dali University, Dali, China.
Biomed Chromatogr. 2022 Apr;36(4):e5320. doi: 10.1002/bmc.5320. Epub 2022 Jan 27.
Polygonati Rhizoma (Huangjing) is traditional medicine in China, which can only be used as medicine after being processed. However, there is a limited theoretical basis for analyzing the changes in chemical components after traditional processing. In this study, analytical techniques including Fourier transform infrared spectroscopy, high-performance gel permeation chromatography-evaporative light scattering detection, and HPLC-diode array detection were proposed to perform multiple fingerprint analyses of the changes in the processed materials; the total sugar was also determined. Moreover, the chemometric studies, including hierarchical cluster analysis and principal component analysis, were used to visualize the discrimination of raw and processed materials. The results revealed that the chemical constituents had been profoundly changed following sample processing. In conclusion, these methods could be successfully used to compare raw and processed materials of Polygonatum kingianum, which could be used to elaborate the rationality of processing from the perspective of chemical composition.
黄精（Polygonati Rhizoma）是中国传统药材，需经过炮制后才可入药。然而，目前对于传统炮制前后化学成分变化的分析缺乏理论依据。本研究采用傅里叶变换红外光谱、高效凝胶渗透色谱-蒸发光散射检测和高效液相色谱-二极管阵列检测等分析技术，对炮制前后物料的变化进行了多指标指纹图谱分析，并测定了总糖含量。同时，采用层次聚类分析和主成分分析等化学计量学方法对生品和炮制品进行直观判别。结果表明，样品炮制后化学组成发生了显著变化。综上所述，该方法可用于比较玉竹的生品和炮制品，从化学成分的角度阐释炮制的合理性。
