School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China.
Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, 19 Chlorine Gardens, Belfast BT9 5DL, United Kingdom.
J Agric Food Chem. 2022 Jan 19;70(2):458-469. doi: 10.1021/acs.jafc.1c06808. Epub 2022 Jan 5.
Rapid and sensitive detection technologies for food contaminants play vital roles in food safety. Due to the complexity of the food matrix and the trace amount distribution, traditional methods often suffer from unsatisfying accuracy, sensitivity, or specificity. In past decades, single-molecule detection (SMD) has emerged as a way to realize the rapid and ultrasensitive measurement with low sample consumption, showing a great potential in food contaminants detection. For instance, based on the nanopore technique, simple and effective methods for single-molecule analysis of food contaminants have been developed. To our knowledge, there has been a rare review that focuses on SMD techniques for food safety. The present review attempts to cover some typical SMD methods in food safety, including electrochemistry, optical spectrum, and atom force microscopy. Then, recent applications of these techniques for detecting food contaminants such as biotoxins, pesticides, heavy metals, and illegal additives are reviewed. Finally, existing research challenges and future trends of SMD in food safety are also tentatively proposed.
快速灵敏的食品污染物检测技术在食品安全中起着至关重要的作用。由于食品基质的复杂性和痕量分布，传统方法往往在准确性、灵敏度或特异性方面不尽如人意。在过去的几十年中，单分子检测（SMD）已经成为一种以低样品消耗实现快速和超高灵敏度测量的方法，在食品污染物检测方面显示出巨大的潜力。例如，基于纳米孔技术，已经开发出用于食品污染物单分子分析的简单有效的方法。据我们所知，很少有综述专门关注食品安全的 SMD 技术。本综述试图涵盖食品安全中的一些典型 SMD 方法，包括电化学、光学光谱和原子力显微镜。然后，综述了这些技术在检测生物毒素、农药、重金属和非法添加剂等食品污染物方面的最新应用。最后，还初步提出了食品安全中单分子检测目前的研究挑战和未来趋势。
