School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Anal Chem. 2022 Jan 18;94(2):1076-1084. doi: 10.1021/acs.analchem.1c04076. Epub 2022 Jan 5.
Closely related to multiple chronic inflammation, especially type-2 diabetes (T2D), methylglyoxal (MGO) may be a potential key to visualize disease progression and treatment effects. On the other hand, lack of convenient and fast analytical methods cannot afford accurate MGO quantitative evaluation. In this work, an activatable second near-infrared region (NIR-II) fluorescent probe TDTCD was synthesized and its reaction mechanism with MGO was discussed. The desired NIR-II product preferred response solvents with small polarity. A novel activatable nanoprobe, MG-SLNP, for MGO was then constructed based on rational packaging to provide a local nonpolar microenvironment. The hydrophobic core of nanoparticles not only successfully improved the stability and water solubility but also greatly promoted the response rate while reacting with MGO. The comparison between NIR-II fluorescence and the traditional high-performance liquid chromatography method for T2D blood samples was discussed. A high-resolution viewing window, quick response, and good biocompatibility led to a satisfactory signal-to-noise ratio of MG-SLNP for real-time MGO bio-detection and imaging in vivo.
与多种慢性炎症密切相关，特别是 2 型糖尿病 (T2D)，甲基乙二醛 (MGO) 可能是可视化疾病进展和治疗效果的潜在关键。另一方面，缺乏方便快捷的分析方法，无法进行准确的 MGO 定量评估。在这项工作中，我们合成了一种可激活的近红外二区 (NIR-II) 荧光探针 TDTCD，并讨论了其与 MGO 的反应机制。所需的 NIR-II 产物优先选择极性较小的反应溶剂。然后，基于合理的封装，构建了一种新型的用于 MGO 的可激活纳米探针 MG-SLNP，以提供局部非极性微环境。纳米颗粒的疏水核不仅成功提高了稳定性和水溶性，而且在与 MGO 反应时大大提高了反应速率。讨论了 NIR-II 荧光与传统高效液相色谱法用于 T2D 血液样本检测的比较。高分辨率观察窗口、快速响应和良好的生物相容性使 MG-SLNP 具有良好的信噪比，可用于实时 MGO 生物检测和体内成像。
