School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, China.
School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, China.
Biosens Bioelectron. 2022 May 15;204:114068. doi: 10.1016/j.bios.2022.114068. Epub 2022 Feb 5.
Methylglyoxal (MGO), a glycolysis metabolite with high reactivity, can nonenzymatically modify proteins, lipids and nucleic acids etc., and it is closely related to the development of tumors. The accurate detection and high-performance optical imaging of MGO from deep tumor issues is of great significance for understanding their roles in tumor initiation and progression. Herein, we have presented a nanoprobe D/I-PNTs with emission in the first near infrared (NIR-I) region by employing a fluorescence resonance energy transfer (FRET) process between a far-red emission MGO probe and IR783 based on peptide nanotubes. The nanoplatform extended the emission range of MGO probe through FRET process and avoided complex molecular design and synthesis. The biocompatible peptide nanotubes improved the water solubility of MGO probe. D/I-PNTs was sensitive to MGO with a detection limit of 272 nM and enabled high-resolution NIR-I fluorescence imaging of MGO induced by glyoxalase I (GLO1) inhibitor in tumor with higher penetration depth (∼4 mm) than that in visible (Vis) region (∼3 mm). Most importantly, the FRET process based on the structure characteristics of peptide nanotubes can be a universal approach to realize the extension of emission wavelength and ratio detection of target analytes, which will be a promising strategy for bioimaging in deep tissue with high contrast.
甲基乙二醛（MGO）是一种具有高反应性的糖酵解代谢物，可以非酶促修饰蛋白质、脂质和核酸等，与肿瘤的发展密切相关。从深部肿瘤问题中准确检测和高性能光学成像 MGO，对于了解其在肿瘤起始和进展中的作用具有重要意义。在此，我们通过基于肽纳米管的远红发射 MGO 探针和 IR783 之间的荧光共振能量转移（FRET）过程，展示了一种发射在近红外一区（NIR-I）的纳米探针 D/I-PNTs。该纳米平台通过 FRET 过程扩展了 MGO 探针的发射范围，避免了复杂的分子设计和合成。生物相容性肽纳米管提高了 MGO 探针的水溶性。D/I-PNTs 对 MGO 具有高灵敏度，检测限为 272 nM，并能够在比可见区（Vis）更深的深度（约 4 mm）对 GLO1 抑制剂诱导的 MGO 进行高分辨率 NIR-I 荧光成像，而 Vis 区的深度约为 3 mm。最重要的是，基于肽纳米管结构特征的 FRET 过程可以成为实现发射波长扩展和目标分析物比率检测的通用方法，这将是深部组织高对比度生物成像的一种有前途的策略。
