Department of Chemistry, American University of Beirut, Beirut, Lebanon.
Luminescence. 2022 Mar;37(3):422-430. doi: 10.1002/bio.4185. Epub 2022 Jan 17.
One of the limitations of fluorescence probe molecules during biomedical estimation is their lack of ability to selectively determine the targeted species. To overcome this there have been various approaches that involve attaching a functional group or aptamers to the fluorescence probe. However, encapsulating probe molecules in a matrix using nanotechnology can be a viable and easier method. Curcumin (Cur) as a fluorescence marker cannot distinguish DNA and RNA. This research reports a novel selective approach involving the use of nanocapsules composed of liposomal curcumin coated with chitosan for the selective detection of RNA molecules using a fluorescence method. The increase in RNA concentration enhanced the electrostatic interaction between the negatively charge surface of RNA and the positively charged nanocapsule, which was further verified by zeta potential measurement. This method had a low limit of detection (36 ng/ml) and higher linear dynamic ranges compared with other studies found in the literature. Moreover, the method was not affected by DNA and was selective for the detection of RNA molecules for which the site of interaction was confined only to uracil. The selectivity for RNA molecules towards other analogues species was also examined and recovery range found was between 99 and 100.33%.
荧光探针分子在生物医学评估中的一个局限性是它们缺乏选择性地确定目标物种的能力。为了克服这一问题，已经有了各种方法，包括将官能团或适体附着到荧光探针上。然而，使用纳米技术将探针分子封装在基质中可能是一种可行且更容易的方法。姜黄素 (Cur) 作为荧光标记物无法区分 DNA 和 RNA。这项研究报告了一种新的选择性方法，涉及使用由脂质体姜黄素涂覆壳聚糖组成的纳米胶囊，通过荧光法选择性检测 RNA 分子。随着 RNA 浓度的增加，增强了带负电荷的 RNA 表面和带正电荷的纳米胶囊之间的静电相互作用，这通过zeta 电位测量进一步得到证实。与文献中其他研究相比，该方法具有较低的检测限（36ng/ml）和更高的线性动态范围。此外，该方法不受 DNA 的影响，并且对 RNA 分子具有选择性，其相互作用的部位仅限于尿嘧啶。还检查了 RNA 分子对其他类似物的选择性，发现回收率在 99%至 100.33%之间。
