Department of Chemistry, American University of Beirut, PO Box 11-0236, Riad El Solh, Beirut, 1107 2020, Lebanon.
J Fluoresc. 2020 May;30(3):547-556. doi: 10.1007/s10895-020-02528-9. Epub 2020 Mar 20.
In a novel approach, curcumin has been encapsulated inside Poly(Ethylene Oxide)-Block-Poly(Propylene Oxide)-Block-Poly(Ethylene Oxide) (F108) nanocapsules. FTIR spectra have indicated a type of hydrogen bonding and dipole interaction between curcumin and F108. Fluorescence and UV-visible absorption profiles of curcumin in nanocapsules have indicated location of curcumin in more hydrophobic microenvironment. The relative fluorescence yield has increased by 6 times in the nanocapsules, which renders them as more sensitive probes to be used later on in sensing study. Therefore, based on the functionality of curcumin as a fluorescent transducer, encapsulated curcumin is used in biomedical application as DNA and RNA sensing. Detection limits are detected as 50 μM and 60 μM for DNA and RNA respectively. Linear dynamic concentration range obtained in this proposed method is much higher than reported in literature. The interaction between the nanocapsules and targeted DNA/RNA molecules is further approved by zeta potential studies. Furthermore, the real interaction of DNA with the encapsulated curcumin is confirmed by the interaction of the adenine and cytosine nucleotides. This has been verified through zeta potential measurements. Moreover, our prepared nanocapsules has presented a high percentage recovery of DNA and RNA (96-101%). Finally, stability results have illustrated a high photostability of encapsulated curcumin, indicating that proposed nanocapsules can be considered as a stable sensor during measurement time.
在一种新颖的方法中，姜黄素被包裹在聚（环氧乙烷）-嵌段-聚（环氧丙烷）-嵌段-聚（环氧乙烷）（F108）纳米胶囊内。傅里叶变换红外光谱表明姜黄素与 F108 之间存在氢键和偶极相互作用。纳米胶囊中姜黄素的荧光和紫外-可见吸收谱表明姜黄素位于更疏水的微环境中。纳米胶囊中姜黄素的相对荧光产率增加了 6 倍，这使得它们成为更灵敏的探针，可用于后续的传感研究。因此，基于姜黄素作为荧光传感器的功能，包裹的姜黄素被用于生物医学应用，如 DNA 和 RNA 传感。分别检测到 DNA 和 RNA 的检测限为 50 μM 和 60 μM。与文献报道相比，本方法获得的线性动态浓度范围要高得多。纳米胶囊与靶向 DNA/RNA 分子的相互作用通过ζ电位研究得到进一步证实。此外，通过ζ电位测量证实了纳米胶囊与封装的姜黄素之间的 DNA 实际相互作用。此外，我们制备的纳米胶囊对 DNA 和 RNA 的回收率高达 96-101%。最后，稳定性结果表明，封装的姜黄素具有很高的光稳定性，表明在测量过程中，所提出的纳米胶囊可以作为一种稳定的传感器。
