COVID-19 和 SARS-CoV-2 对男性生殖功能的影响：系统评价和荟萃分析方案。
Impacts of COVID-19 and SARS-CoV-2 on male reproductive function: a systematic review and meta-analysis protocol.
机构信息
Gynecology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, People's Republic of China.
School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, People's Republic of China.
出版信息
BMJ Open. 2022 Jan 5;12(1):e053051. doi: 10.1136/bmjopen-2021-053051.
INTRODUCTION
COVID-19 pandemic caused by SARS-CoV-2 has become a global health challenge. SARS-CoV-2 can infect host cells via the ACE2 receptor, which is widely expressed in the corpus cavernosum, testis and male reproductive tract, and participates in erection, spermatogenesis and androgen metabolism. Also, the immune response and persistent fever resulting from COVID-19 may lead to damage of the testicular activity, consequently compromising male fertility.
METHODS AND ANALYSIS
PubMed, MEDLINE, EMBASE, Web of Science, Cochrane Library, China National Knowledge Infrastructure, China Science and Technology Journal database, Chinese Biomedical Databases and Wanfang Data will be systematically searched for observational studies (case-control and cohort) published up to March 2021 in English or in Chinese literature on the impacts of COVID-19 and SARS-CoV-2 on male reproductive function. This protocol will follow the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols guidelines and Meta-analysis of Observational Studies in Epidemiology. The primary outcome will be semen parameters, and the additional outcomes will include: (a) detection of SARS-COV-2 in semen, (b) male sexual hormones, (c) sperm DNA fragmentation index, (d) erectile function, (e) evaluation of testis and also the male genital tract. Two reviewers will independently extract data from the included studies based on a predesigned data extraction form. The risk of bias of included studies will be evaluated through the Newcastle-Ottawa Scale for observational studies. Review Manager software V.5.3 will be used for statistical analysis. Q statistic and I² test will be performed to assess the heterogeneity among studies. Sensitivity analysis will be used to explore the robustness of pooled effects. We will use the Grading of Recommendations Assessment, Development and Evaluation system to assess the quality of evidence.
ETHICS AND DISSEMINATION
Ethical approval is not required and results will be published in a peer-reviewed journal.
PROSPERO REGISTRATION NUMBER
CRD42021245161.
简介
由 SARS-CoV-2 引起的 COVID-19 大流行已成为全球健康挑战。SARS-CoV-2 可以通过 ACE2 受体感染宿主细胞，ACE2 受体在阴茎海绵体、睾丸和男性生殖道中广泛表达，并参与勃起、精子发生和雄激素代谢。此外，COVID-19 引起的免疫反应和持续发热可能导致睾丸活动损伤，从而影响男性生育能力。
方法和分析
我们将系统地检索 PubMed、MEDLINE、EMBASE、Web of Science、Cochrane 图书馆、中国国家知识基础设施、中国科技期刊数据库、中国生物医学文献数据库和万方数据，以获取截至 2021 年 3 月发表的关于 COVID-19 和 SARS-CoV-2 对男性生殖功能影响的英文或中文文献中的观察性研究（病例对照和队列研究）。本方案将遵循系统评价和荟萃分析报告规范以及流行病学观察性研究荟萃分析的建议。主要结局指标为精液参数，次要结局指标包括：（a）精液中 SARS-COV-2 的检测，（b）男性性激素，（c）精子 DNA 碎片化指数，（d）勃起功能，（e）睾丸和男性生殖器官的评估。两位评审员将根据预先设计的数据提取表独立提取纳入研究的数据。通过 Newcastle-Ottawa 量表评估纳入研究的偏倚风险。使用 Review Manager 软件 V.5.3 进行统计分析。使用 Q 统计量和 I² 检验评估研究之间的异质性。进行敏感性分析以探索汇总效应的稳健性。我们将使用推荐评估、制定与评估系统（Grading of Recommendations Assessment, Development and Evaluation system）评估证据质量。
伦理与传播
本研究无需伦理批准，研究结果将发表在同行评议的期刊上。
PROSPERO 注册号：CRD42021245161。
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