Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Parasitology and Mycology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Comp Immunol Microbiol Infect Dis. 2022 Feb;81:101720. doi: 10.1016/j.cimid.2021.101720. Epub 2021 Nov 18.
In this study, we investigated the possible association between TB and Toxoplasma gondii infection. One hundred confirmed TB individuals living in northwest Iran were classified into three subgroups; newly diagnosed patients (NTB), old diagnosed patients (OTB) and multidrug resistance patients (MDR-TB). One hundred healthy subjects in the same age and sex distribution were ethnically matched. Sera samples were screened for anti-Toxoplasma antibodies. Nested-PCR was performed by targeting the B1 and GRA6 genes. The frequency of Toxoplasma infection based on IgG titer was 71.1% in the OTB subgroup and 33% in the control group, indicating significant association between Toxoplasma seropositivity and OTB (P = 0.001). According to phylogenetic network, the type I strain of Toxoplasma was identified in the OTB subgroup (10.1%). We concluded that patients with OTB subgroup are at high risk for acquisition of Toxoplasma infection which could reactivate the latent toxoplasmosis.
在这项研究中，我们调查了结核病（TB）和刚地弓形虫感染之间可能存在的关联。100 名居住在伊朗西北部的确诊 TB 患者被分为三组：新诊断患者（NTB）、旧诊断患者（OTB）和耐多药结核病（MDR-TB）患者。在相同的年龄和性别分布中，选择 100 名健康受试者作为对照组。对血清样本进行抗弓形虫抗体筛查。通过针对 B1 和 GRA6 基因进行巢式 PCR。根据 IgG 滴度，OTB 亚组的弓形虫感染频率为 71.1%，对照组为 33%，表明弓形虫血清阳性与 OTB 之间存在显著关联（P=0.001）。根据系统发育网络，OTB 亚组中鉴定出了 I 型刚地弓形虫（10.1%）。我们得出结论，OTB 亚组的患者感染弓形虫的风险较高，可能会使潜伏性弓形体病重新激活。
