Du Ting, Lu Jian, Liu Lingzhi, Dong Nan, Fang Liurong, Xiao Shaobo, Han Heyou
State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
ACS Appl Bio Mater. 2018 Nov 19;1(5):1286-1293. doi: 10.1021/acsabm.8b00154. Epub 2018 Nov 8.
Developing nanomaterials-based antimicrobial agents has shown a widespread promise. In this study, silver nanoparticle-modified graphene oxide (GO-AgNPs) nanocomposites were self-assembled via interfacial electrostatic force. By using the porcine reproductive and respiratory syndrome virus (PRRSV) as a pattern, the antiviral effect of the as-prepared GO-AgNPs nanocomposites on the replication of virus was investigated. The results indicated that exposure with GO-AgNPs nanocomposites could obviously suppress PRRSV infection. It was found that GO-AgNPs nanocomposites exhibited a better inhibitory effect compared with AgNPs and GO. By selecting the porcine epidemic diarrhea virus (PEDV) as a contrast virus, GO-AgNPs nanocomposites were proven to have a broad antiviral activity. Mechanism studies showed that GO-AgNPs nanocomposites might prevent PRRSV from entering the host cells, with 59.2% inhibition efficiency. Meanwhile, GO-AgNPs nanocomposite treatment enhances the production of interferon-α (IFN-α) and IFN-stimulating genes (ISGs), which can directly inhibit the proliferation of virus. Taken together, this study reports a new type of antiviral agent and provides a promising pharmaceutical agent for treating infection by the highly pathogenic PRRSV. Moreover, it may provide novel ideas for the research and development of antiviral formulations based on nanocomposites and extend their applications in biological systems.
开发基于纳米材料的抗菌剂已展现出广泛的前景。在本研究中，银纳米颗粒修饰的氧化石墨烯（GO-AgNPs）纳米复合材料通过界面静电力自组装而成。以猪繁殖与呼吸综合征病毒（PRRSV）为模型，研究了所制备的GO-AgNPs纳米复合材料对病毒复制的抗病毒效果。结果表明，用GO-AgNPs纳米复合材料处理可明显抑制PRRSV感染。发现GO-AgNPs纳米复合材料与AgNPs和GO相比表现出更好的抑制效果。通过选择猪流行性腹泻病毒（PEDV）作为对照病毒，证明GO-AgNPs纳米复合材料具有广泛的抗病毒活性。机制研究表明，GO-AgNPs纳米复合材料可能阻止PRRSV进入宿主细胞，抑制效率为59.2%。同时，GO-AgNPs纳米复合材料处理可增强干扰素-α（IFN-α）和干扰素刺激基因（ISGs）的产生，它们可直接抑制病毒增殖。综上所述，本研究报道了一种新型抗病毒剂，为治疗高致病性PRRSV感染提供了一种有前景的药剂。此外，它可能为基于纳米复合材料的抗病毒制剂的研发提供新思路，并扩展其在生物系统中的应用。
