Basu Alex, Celma Gunta, Strømme Maria, Ferraz Natalia
Nanotechnology and Functional Materials, Department of Engineering Sciences, Uppsala University, Box 534, 751 21 Uppsala, Sweden.
ACS Appl Bio Mater. 2018 Dec 17;1(6):1853-1863. doi: 10.1021/acsabm.8b00370. Epub 2018 Nov 21.
Current trends in wound care research move toward the development of wound healing dressings designed to treat different types of wounds (e.g., burns and chronic wounds) and toward tailoring treatments for different stages of the wound healing process. In this context, the development of advanced nanotherapeutic materials is highlighted as a promising strategy to efficiently control specific phases of the wound healing process. Here, Ca-cross-linked wood-derived nanofibrillated cellulose (NFC) hydrogels are evaluated as wound healing dressings. In vitro biocompatibility assays were performed to study the interaction of the NFC hydrogels with cellular processes that are tightly related to wound healing. Moreover, an in vivo dermo-epidermic full thickness wound healing model in rat was used to uncover the wound healing ability of the Ca-cross-linked NFC hydrogels. The in vitro experiments showed that the NFC hydrogels were able to support fibroblast and keratinocyte proliferation. A potential effect of the hydrogels on triggering keratinocyte differentiation was furthermore proposed. In vivo, the NFC hydrogels stimulated healing without causing any adverse local tissue effects, potentially owing to their moisture-donating properties and the herein discussed aiding effect of the Ca-cross-linker on epidermal generation. Thus, this work extensively demonstrates the wound healing ability of NFC hydrogels and presents an important milestone in the research on NFC toward advanced wound healing applications.
伤口护理研究的当前趋势朝着开发旨在治疗不同类型伤口（如烧伤和慢性伤口）的伤口愈合敷料发展，并朝着为伤口愈合过程的不同阶段量身定制治疗方法发展。在这种背景下，先进的纳米治疗材料的开发被视为有效控制伤口愈合过程特定阶段的一种有前景的策略。在此，钙交联的木质来源纳米原纤化纤维素（NFC）水凝胶被评估为伤口愈合敷料。进行了体外生物相容性测定，以研究NFC水凝胶与与伤口愈合密切相关的细胞过程之间的相互作用。此外，使用大鼠体内真皮 - 表皮全层伤口愈合模型来揭示钙交联NFC水凝胶的伤口愈合能力。体外实验表明，NFC水凝胶能够支持成纤维细胞和角质形成细胞的增殖。此外，还提出了水凝胶对触发角质形成细胞分化的潜在作用。在体内，NFC水凝胶促进愈合而不引起任何局部组织不良影响，这可能归因于它们的保湿特性以及本文讨论的钙交联剂对表皮生成的辅助作用。因此，这项工作广泛证明了NFC水凝胶的伤口愈合能力，并在NFC用于先进伤口愈合应用的研究中迈出了重要的里程碑。
