Wei Chunbo, Zhao Shengnan, Zhang Yuntao, Gu Wenbo, Kumar Sarker Shuvan, Liu Shuande, Li Benzhang, Wang Xuanyang, Li Ying, Wang Xu
National Key Discipline, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin, China.
Department of Neurosurgery, The 962nd Hospital of the PLA Joint Logistic Support Force, Harbin, China.
Front Nutr. 2021 Dec 23;8:740741. doi: 10.3389/fnut.2021.740741. eCollection 2021.
This study explored the effect of multiple-nutrient supplementation on muscle damage and liver and kidney function after vigorous exercise under heat. After an initial pilot trial comprising 89 male participants, 85 participants were recruited and assigned into three groups: a multiple-nutrient (M) group, a glucose (G) group, and a water (W) group. Multiple-nutrient supplements contain glucose, fructose, maltose, sodium, potassium, vitamin B, vitamin B, vitamin C, vitamin K, and taurine. Participants were organised to take a 3-km running test (wet-bulb globe temperature 32°C) after a short-term (7 days) supplement. Blood samples were obtained to detect biochemical parameters [glucose (GLU), aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN), uric acid (UA), creatinine (Cr), creatine kinase (CK), lactate dehydrogenase (LDH), and lactic acid], inflammation factors [interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α)], and oxidative stress biomarkers [superoxide dismutase (SOD) and 8-iso-prostaglandin F (2alpha) (8-iso-PGF2α)]. In the pilot trial, BUN decreased significantly in the M and G groups immediately after the running test. AST, Cr, and UA were significantly reduced 24 h after the running test with single-shot multiple-nutrient supplementation. In the short-term trial, multiple nutrients further prevented the elevation of CK ( = 0.045) and LDH ( = 0.033) levels 24 h after strenuous exercise. Moreover, we found that multiple nutrients significantly reduced IL-6 ( = 0.001) and TNF-α ( = 0.015) elevation immediately after exercise. Simultaneously, SOD elevation was significantly higher in the M group immediately after exercising than in the other two groups ( = 0.033). 8-iso-PGF2α was reduced in the M group 24 h after exercise ( = 0.036). This study found that multiple-nutrient supplementation promoted the recovery of muscle damage and decreased liver and kidney function caused by strenuous exercise in a hot environment, probably through the inhibition of secondary damage induced by increased inflammatory reactions and oxidative stress. In this respect, the current study has important implications for the strategy of nutritional support to accelerate recovery and potentially prevent heat-related illness. This study was prospectively registered on clinicaltrials.gov on June 21, 2019 (ID: ChiCTR1900023988).
本研究探讨了多种营养素补充对热环境下剧烈运动后肌肉损伤及肝肾功能的影响。在一项包含89名男性参与者的初步预试验之后，招募了85名参与者并将其分为三组：多种营养素（M）组、葡萄糖（G）组和水（W）组。多种营养素补充剂包含葡萄糖、果糖、麦芽糖、钠、钾、维生素B、维生素B、维生素C、维生素K和牛磺酸。在短期（7天）补充后，组织参与者进行3公里跑步测试（湿球黑球温度32°C）。采集血样以检测生化参数[葡萄糖（GLU）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、血尿素氮（BUN）、尿酸（UA）、肌酐（Cr）、肌酸激酶（CK）、乳酸脱氢酶（LDH）和乳酸]、炎症因子[白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）]以及氧化应激生物标志物[超氧化物歧化酶（SOD）和8-异前列腺素F（2α）（8-异-PGF2α）]。在预试验中，跑步测试后M组和G组的BUN立即显著降低。单次补充多种营养素后，跑步测试24小时后AST、Cr和UA显著降低。在短期试验中，多种营养素进一步预防了剧烈运动24小时后CK（P = 0.045）和LDH（P = 0.033）水平的升高。此外，我们发现多种营养素显著降低了运动后即刻IL-6（P = 0.001）和TNF-α（P = 0.015）的升高。同时，运动后即刻M组SOD的升高显著高于其他两组（P = 0.033）。运动24小时后M组8-异-PGF2α降低（P = 0.036）。本研究发现，多种营养素补充可能通过抑制炎症反应和氧化应激增加所诱导的继发性损伤，促进了热环境下剧烈运动所致肌肉损伤的恢复，并减轻了肝肾功能损害。在这方面，本研究对于加速恢复及潜在预防热相关疾病的营养支持策略具有重要意义。本研究于2019年6月21日在clinicaltrials.gov上进行了前瞻性注册（标识符：ChiCTR1900023988）。
