Ugalde-Arbizu Maider, Aguilera-Correa John Jairo, Mediero Aranzazu, Esteban Jaime, Páez Paulina L, San Sebastian Eider, Gómez-Ruiz Santiago
Departamento de Química Aplicada, Facultad de Química, Euskal Herriko Unibertsitatea (UPV/EHU), Paseo Manuel Lardizabal 3, 20018 San Sebastián, Spain.
Clinical Microbiology Department, IIS-Fundación Jiménez Diaz, UAM, Avenida Reyes Católicos 2, 28040 Madrid, Spain.
Pharmaceuticals (Basel). 2022 Jul 18;15(7):884. doi: 10.3390/ph15070884.
(PA) is one of the most common bacteria isolated from chronic wounds and burns. Its treatment is a challenge due to antimicrobial drug resistance and biofilm formation. In this context, this study aimed to perform the synthesis and full characterization of hybrid nanosystems based on mesoporous silica nanoparticles (MSNs) functionalized with a nicotinic ligand and silver chloride nanoparticles, both phenytoin sodium (Ph)-loaded and unloaded, to evaluate the antibacterial properties against three different strains of PA (including two clinical strains) in a planktonic state and as biofilms. Ph is a well-known proliferative agent, which was incorporated into the hybrid nanomaterials to obtain an effective material for tissue healing and prevention of infection caused by PA. The Ph-loaded materials promoted a quasi-complete inhibition of bacterial growth in wound-like medium and biofilm development, with values of 99% and 96%, respectively, with selectivity indices above the requirements for drugs to become promising agents for the topic preventive treatment of chronic wounds and burns.
铜绿假单胞菌（PA）是从慢性伤口和烧伤中分离出的最常见细菌之一。由于其对抗菌药物的耐药性和生物膜形成，其治疗具有挑战性。在此背景下，本研究旨在合成并全面表征基于用烟碱配体功能化的介孔二氧化硅纳米颗粒（MSN）和氯化银纳米颗粒的杂化纳米系统，这些纳米系统分别负载和未负载苯妥英钠（Ph），以评估其对三种不同PA菌株（包括两种临床菌株）在浮游状态和生物膜状态下的抗菌性能。Ph是一种著名的增殖剂，将其掺入杂化纳米材料中以获得一种有效的材料，用于组织愈合和预防PA引起的感染。负载Ph的材料在伤口样培养基中促进了对细菌生长的近乎完全抑制以及生物膜形成，抑制率分别为99%和96%，其选择性指数高于药物成为慢性伤口和烧伤局部预防性治疗有前景药物的要求。
