CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.
Comp Biochem Physiol C Toxicol Pharmacol. 2019 Aug;222:1-10. doi: 10.1016/j.cbpc.2019.04.006. Epub 2019 Apr 11.
Aflatoxin B1 (AFB1) is a mycotoxin mainly produced by Aspergillus flavus and Aspergillus parasiticus contaminating food, feed ingredients and products of animal origin. In mammals, this toxin causes widespread organ-specific damage; it is immunotoxicity and could promote hepatotoxicity, alter intestinal functions and so on. In this study, we conducted transcriptome and histomorphology analyses of hepatopancreas and intestinal in Litopenaeus vannamei (L. vannamei) challenged with AFB1. Totally 12,014 and 1387 differentially expression genes (DEGs) were identified in the hepatopancreas and intestine, respectively. In hepatopancreas, a total of 1995 DEGs were mainly annotated and grouped into 18 processes or pathways related to animal immune system. With respect to intestine, a total of 152 DEGs were mainly annotated to 7 processes or pathways related to animal immune system. Meanwhile, we determined the relative mRNA expression of several crucial representative immune genes including Toll, immune deficiency (IMD), prophenoloxidase (proPO), Rab and glutathione S-transferase (GST) in the hepatopancreas and intestines of shrimp at 3-, 6-, 12-, 18-, 24- and 30-d after challenged by AFB1. Exposure to AFB1 increased mortality, decrease weight gain rate, severely destroyed the histomorphology of hepatopancreas and intestine, and resulted in the damaged of immune system of shrimp. The present data reveals the different roles between hepatopancreas and intestine of L. vannamei in immune response to AFB1 challenge, and provides insight into the molecular basis of the relationship between hepatopancreas and intestinal immunity during either homeostasis or inflammation.
黄曲霉毒素 B1（AFB1）是一种主要由黄曲霉和寄生曲霉产生的真菌毒素，污染食物、饲料成分和动物源性产品。在哺乳动物中，这种毒素会导致广泛的器官特异性损伤；它具有免疫毒性，并可能促进肝毒性、改变肠道功能等。在这项研究中，我们对凡纳滨对虾（L. vannamei）的肝胰腺和肠道进行了转录组和组织形态学分析，以应对 AFB1 的挑战。在肝胰腺和肠道中分别鉴定出 12014 个和 1387 个差异表达基因（DEGs）。在肝胰腺中，共有 1995 个 DEGs 主要被注释并分为 18 个与动物免疫系统相关的过程或途径。对于肠道，共有 152 个 DEGs 主要注释为 7 个与动物免疫系统相关的过程或途径。同时，我们在虾肝胰腺和肠道中测定了几个关键代表免疫基因的相对 mRNA 表达，包括 Toll、免疫缺陷（IMD）、酚氧化酶原（proPO）、Rab 和谷胱甘肽 S-转移酶（GST），在虾肝胰腺和肠道中 3、6、12、18、24 和 30 天后受到 AFB1 的挑战。暴露于 AFB1 会增加死亡率，降低增重率，严重破坏肝胰腺和肠道的组织形态，导致虾免疫系统受损。本数据揭示了凡纳滨对虾肝胰腺和肠道在应对 AFB1 挑战时的免疫反应中的不同作用，并深入了解了在稳态或炎症期间肝胰腺和肠道免疫之间关系的分子基础。
