Brandão-Lima Luciano C, Silva Fabrícia C, Costa Paulo V C G, Alves-Júnior Edgar A, Viseras César, Osajima Josy A, Bezerra Leilson R, de Moura Jose F P, de A Silva Aline G, Fonseca Maria G, Silva-Filho Edson C
Interdisciplinary Laboratory for Advanced Materials-LIMAV, Federal Unviersity of Piauí, Teresina 64049-550, Brazil.
Campus Senador Helvídio Nunes Barros, CSHNB, Federal Unviersityof Piauí, Picos 64600-000, Brazil.
Materials (Basel). 2021 Dec 22;15(1):64. doi: 10.3390/ma15010064.
The potential use of amino acids by ruminal microorganisms converting them into microbial protein for ruminants makes it challenging to supplement these nutrients in an accessible form in animals' diets. Several strategies to protect amino acids from ruminal degradation were reported, producing amino acids available for the protein used in the intestine called "bypass." The intercalation of biomolecules in clay mineral minerals has gained notoriety due to its ability to support, protect, transport, physicochemical properties and non-toxicity. This study aimed to investigate the incorporation of L-lysine (Lys), L-methionine (Met), and L-tryptophan (Trp) amino acids in the clay minerals sepiolite (Sep) and Veegum (Veg) using the adsorption method. The characterization techniques of X-ray diffraction and infrared spectroscopy indicated the presence of biomolecules in the inorganic matrices. Elemental and thermal analyzes monitored the percentages of incorporated amino acids. They showed better incorporation capacities for Veg, such as Met-Veg < Lys-Veg < Trp-Veg and Lys-Sep < Met-Sep < Trp-Sep for sepiolite, except for the incorporation of Met. Matrices provide a promising alternative for planning the administration of biomolecules, using essential amino acids as models, and may offer an alternative to improve functional diet strategies.
瘤胃微生物将氨基酸转化为反刍动物的微生物蛋白，这使得以动物日粮中易获取的形式补充这些营养素具有挑战性。据报道，有几种保护氨基酸不被瘤胃降解的策略，可产生用于肠道蛋白质的可利用氨基酸，即“过瘤胃”氨基酸。生物分子嵌入粘土矿物因其具有支持、保护、运输、物理化学性质及无毒等能力而受到关注。本研究旨在采用吸附法研究L-赖氨酸（Lys）、L-蛋氨酸（Met）和L-色氨酸（Trp）在粘土矿物海泡石（Sep）和维古胶（Veg）中的掺入情况。X射线衍射和红外光谱表征技术表明无机基质中存在生物分子。元素分析和热分析监测了掺入氨基酸的百分比。结果显示，Veg具有更好的掺入能力，如对于海泡石，Met-Veg < Lys-Veg < Trp-Veg，Lys-Sep < Met-Sep < Trp-Sep，但Met的掺入情况除外。这些基质为以必需氨基酸为模型规划生物分子给药提供了一种有前景的选择，并且可能为改进功能性日粮策略提供一种替代方案。
