Pleva Pavel, Bartošová Lucie, Máčalová Daniela, Zálešáková Ludmila, Sedlaříková Jana, Janalíková Magda
Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlin, 275 Vavreckova, 76001 Zlin, Czech Republic.
Department of Food Technology, Faculty of Technology, Tomas Bata University in Zlin, nam. T. G. Masaryka 5555, 76001 Zlin, Czech Republic.
Foods. 2021 Dec 21;11(1):2. doi: 10.3390/foods11010002.
Biofilm is a structured community of microorganisms adhering to surfaces of various polymeric materials used in food packaging. Microbes in the biofilm may affect food quality. However, the presence of biofilm can ensure biodegradation of discarded packaging. This work aims to evaluate a biofilm formation on the selected biodegradable polymer films: poly (lactic acid) (PLA), poly (butylene adipate-co-terephthalate) (PBAT), and poly (butylene succinate) (PBS) by selected bacterial strains; collection strains of , ; and , , , and isolated from dairy products. Three different methods for biofilm evaluation were performed: the Christensen method, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and fluorescence microscopy. High biofilm formation was confirmed on the control PBS film, whereas low biofilm formation ability was observed on the PLA polymer sample. Furthermore, the films with incorporated antimicrobial compounds (thymol or eugenol) were also prepared. Antimicrobial activity and also reduction in biofilm formation on enriched polymer films were determined. Therefore, they were all proved to be antimicrobial and effective in reducing biofilm formation. These films can be used to prepare novel active food packaging for the dairy industry to prevent biofilm formation and enhance food quality and safety in the future.
生物膜是附着在食品包装中使用的各种聚合材料表面的微生物结构化群落。生物膜中的微生物可能会影响食品质量。然而，生物膜的存在可以确保废弃包装的生物降解。这项工作旨在评估选定的可生物降解聚合物薄膜上生物膜的形成情况：聚乳酸（PLA）、聚己二酸丁二醇酯-对苯二甲酸丁二醇酯共聚物（PBAT）和聚丁二酸丁二醇酯（PBS），使用选定的细菌菌株进行评估；从乳制品中分离出的 、 ；以及 、 、 和 的收集菌株。采用了三种不同的生物膜评估方法：克里斯滕森方法、3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法和荧光显微镜法。在对照PBS薄膜上证实有高生物膜形成，而在PLA聚合物样品上观察到低生物膜形成能力。此外，还制备了含有抗菌化合物（百里香酚或丁香酚）的薄膜。测定了富集聚合物薄膜上的抗菌活性以及生物膜形成的减少情况。因此，它们都被证明具有抗菌性且能有效减少生物膜形成。这些薄膜可用于制备新型活性食品包装，用于乳制品行业，以防止生物膜形成，并在未来提高食品质量和安全性。
