Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Korea.
Microbiome Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.
Int J Mol Sci. 2022 Jun 17;23(12):6770. doi: 10.3390/ijms23126770.
Owing to the rapid spread of antibiotic resistance among species, effective and low-risk alternatives to antibiotics are being actively searched. Thymol (THO), the most abundant component of the oil extracted from thyme, can be considered as a natural antibacterial alternative. However, the low antibacterial activity and non-selectivity of THO limit its usage as a universal anti- agent. Herein, we report the bioconjugation of THO with ZnO nanoparticle (ZO), which resulted in the TZ nanocomposite (NC), as a potent and selective antibacterial agent against species, particularly . The cell-free supernatant (CFS) of ATCC 25923 cultures was employed for the production of TZ NC. Successful production of TZ NC was confirmed via X-ray diffraction (XRD), Fourier-transform infrared (FT-IR) spectroscopy, and ultraviolet-visible (UV-Vis) studies. TZ NC had selective efficacy against species, with MIC values 2-32-fold lower than THO. The antibacterial mechanisms of TZ NC are proposed to involve membrane rupture, suppression of biofilm formation, and modulation of new cell wall and protein-synthesis-associated cellular pathways. Its biocompatibility against HCT116 cells was also checked. Our findings suggest that the TZ nanocomposite could improve the selectivity and bactericidal activity of THO against target species.
由于抗生素耐药性在 物种中的迅速传播，人们正在积极寻找有效且低风险的抗生素替代品。百里香酚（THO）是从百里香油中提取的最丰富的成分，可以被认为是一种天然的抗菌替代品。然而，THO 的抗菌活性低和非选择性限制了其作为通用抗菌剂的使用。在这里，我们报告了将 THO 与氧化锌纳米粒子（ZO）进行生物共轭，得到 TZ 纳米复合材料（NC），作为一种针对 物种，特别是 的有效和选择性抗菌剂。使用 ATCC 25923 培养物的无细胞上清液（CFS）来生产 TZ NC。通过 X 射线衍射（XRD）、傅里叶变换红外（FT-IR）光谱和紫外可见（UV-Vis）研究证实了 TZ NC 的成功生产。TZ NC 对 物种具有选择性疗效，MIC 值比 THO 低 2-32 倍。TZ NC 的抗菌机制被认为涉及膜破裂、抑制生物膜形成以及调节新的细胞壁和与蛋白质合成相关的细胞途径。还检查了其对 HCT116 细胞的生物相容性。我们的研究结果表明，TZ 纳米复合材料可以提高 THO 对靶物种的选择性和杀菌活性。
Zotero 插件
