The Institute of Plant Sciences, Volcani Institute, ARO, Rishon LeZion 7528809, Israel.
Cells. 2022 Jan 3;11(1):145. doi: 10.3390/cells11010145.
The distribution of myosin VIII ATM1 tail in association with the plasma membrane is often observed in coordination with that of cortical microtubules (MTs). The prevailing hypothesis is that coordination between the organization of cortical MTs and proteins in the membrane results from the inhibition of free lateral diffusion of the proteins by barriers formed by MTs. Since the positioning of myosin VIII tail in the membrane is relatively stable, we ask: can it affect the organization of MTs? Myosin VIII ATM1 tail co-localized with remorin 6.6, the position of which in the plasma membrane is also relatively stable. Overexpression of myosin VIII ATM1 tail led to a larger fraction of MTs with a lower rate of orientation dispersion. In addition, collisions between MTs and cortical structures labeled by ATM1 tail or remorin 6.6 were observed. Collisions between EB1 labeled MTs and ATM1 tail clusters led to four possible outcomes: 1-Passage of MTs through the cluster; 2-Decreased elongation rate; 3-Disengagement from the membrane followed by a change in direction; and 4-retraction. EB1 tracks became straighter in the presence of ATM1 tail. Taken together, collisions of MTs with ATM1 tail labeled structures can contribute to their coordinated organization.
肌球蛋白 VIII ATM1 尾部与质膜的分布经常与皮质微管（MTs）的分布协调观察到。流行的假说是，皮质 MTs 的组织与膜中蛋白质之间的协调是由于 MTs 形成的屏障抑制了蛋白质的自由侧向扩散。由于肌球蛋白 VIII 尾部在膜中的定位相对稳定，我们会问：它会影响 MTs 的组织吗？肌球蛋白 VIII ATM1 尾部与 remorin 6.6 共定位，其在质膜中的位置也相对稳定。肌球蛋白 VIII ATM1 尾部的过表达导致具有较低取向分散率的 MTs 的比例增加。此外，还观察到 MTs 与由 ATM1 尾部或 remorin 6.6 标记的皮质结构之间的碰撞。EB1 标记的 MTs 与 ATM1 尾部簇之间的碰撞有四种可能的结果：1-MTs 通过簇的通过；2-伸长率降低；3-与膜脱离，然后改变方向；4-回缩。在 ATM1 尾部存在的情况下，EB1 轨迹变得更直。总之，MTs 与标记有 ATM1 尾部的结构的碰撞可能有助于它们的协调组织。
