Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan.
Livestock Research Institute, Council of Agriculture, Executive Yuan, Tainan, Taiwan.
Sci Rep. 2022 Jan 10;12(1):445. doi: 10.1038/s41598-021-03855-y.
An appropriate animal wound model is urgently needed to assess wound dressings, cell therapies, and pharmaceutical agents. Minipig was selected owing to similarities with humans in body size, weight, and physiological status. Different wound sizes (0.07-100 cm) were created at varying distances but fail to adequately distinguish the efficacy of various interventions. We aimed to resolve potential drawbacks by developing a systematic wound healing system. No significant variations in dorsal wound closure and contraction were observed within the thoracolumbar region between boundaries of both armpits and the paravertebral region above rib tips; therefore, Lanyu pigs appear suitable for constructing a reliable dorsal wound array. Blood flow signals interfered with inter-wound distances ˂ 4 cm; a distance > 4 cm is therefore recommended. Wound sizes ≥ 4 cm × 4 cm allowed optimal differentiation of interventions. Partial- (0.23 cm) and full-thickness (0.6 cm) wounds showed complete re-epithelialization on days 13 and 18 and strongest blood flow signals at days 4 and 11, respectively. Given histological and tensile strength assessments, tissue healing resembling normal skin was observed at least after 6 months. We established some golden standards for minimum wound size and distance between adjacent wounds for effectively differentiating interventions in considering 3R principles.
为了评估伤口敷料、细胞疗法和药物制剂，我们急需一种合适的动物创伤模型。小型猪因其在体型、体重和生理状态方面与人类相似而被选中。不同大小的伤口（0.07-100 cm）在不同的距离处形成，但未能充分区分各种干预措施的疗效。我们旨在通过开发一种系统性的伤口愈合系统来解决潜在的缺陷。在腋窝之间和肋尖上方的椎旁区域的胸腰椎区域内，背部伤口的闭合和收缩没有明显的差异；因此，兰屿猪似乎适合构建可靠的背部伤口阵列。血流信号会干扰 ˂ 4cm 的伤口间距离；因此建议选择 ˃ 4cm 的距离。伤口大小 ≥4cm×4cm 可以实现干预措施的最佳区分。部分（0.23cm）和全层（0.6cm）伤口在第 13 天和第 18 天分别完全上皮化，在第 4 天和第 11 天分别显示最强的血流信号。根据组织学和拉伸强度评估，在至少 6 个月后，观察到与正常皮肤相似的组织愈合。我们为最小伤口大小和相邻伤口之间的距离建立了一些黄金标准，以考虑到 3R 原则，有效区分干预措施。
