委内瑞拉共流行地区家养、周边地区及野生哺乳动物中克氏锥虫/利什曼原虫属混合感染的分子诊断
Molecular diagnosis of Trypanosoma cruzi/Leishmania spp. coinfection in domestic, peridomestic and wild mammals of Venezuelan co-endemic areas.
作者信息
Viettri Mercedes, Herrera Leidi, Aguilar Cruz M, Morocoima Antonio, Reyes Jesús, Lares María, Lozano-Arias Daisy, García-Alzate Roberto, Chacón Tony, Feliciangeli María D, Ferrer Elizabeth
机构信息
Instituto de Investigaciones Biomédicas "Dr. Francisco J. Triana Alonso" (BIOMED), Universidad de Carabobo Sede Aragua, Maracay, estado Aragua, Venezuela; Departamento de Clinico Integral, Facultad de Ciencias de la Salud, Universidad de Carabobo Sede Aragua, Maracay, estado Aragua, Venezuela.
Instituto de Zoología y Ecología Tropical (IZET), Facultad de Ciencias, Universidad Central de Venezuela (UCV), Caracas, Venezuela.
出版信息
Vet Parasitol Reg Stud Reports. 2018 Dec;14:123-130. doi: 10.1016/j.vprsr.2018.10.002. Epub 2018 Oct 11.
American trypanosomiasis and leishmaniases are diseases caused by protozoans of the Trypanosomatidae family. In Venezuela, although several endemic foci of both diseases coincide, there are no reports of coinfection in mammals. The molecular diagnosis of the coinfection T. cruzi-Leishmania spp. was done in 527 blood samples collected on filter paper of several species of mammals (Canis familiaris, Equus asinus, Didelphis marsupialis, Equus mulus, Rattus rattus, Equus caballus, Artibeus fraterculus, Felis catus, Sus scrofa, Bos taurus, Capra hircus and Sciurus granatensis) from the states Cojedes, Aragua, Anzoátegui, Guárico, Miranda and Capital District. The T. cruzi infection was determined through PCR amplification of DNA of kinetoplast minicircles (kDNA) and satellite DNA (sDNA). The Leishmania spp. infection was detected by Leishmania nested PCR (Ln-PCR), and ribosomal DNA internal transcribed spacer 1 PCR (ITS1-PCR). The percentage of infection by T. cruzi was 23.5%, by Leishmania spp. 12.9% and coinfection was 5.7%. D. marsupialis was the species with the highest percentage of infection for each parasitosis (T. cruzi 34.3%, Leishmania spp. 20.0%) and coinfection (14.3%). Anzoátegui was the state with the highest percentage of infection for each parasitosis (T. cruzi 64.9%, Leishmania spp. 64.9%) and coinfection (43.2%). Infections were determined in species not reported as natural reservoirs of T. cruzi (E. asinus and E. mulus) and of Leishmania spp. (E. mulus and S. scrofa). Coinfection was a frequent phenomenon in mammals in several co-endemic zones evaluated.
美洲锥虫病和利什曼病是由锥虫科原生动物引起的疾病。在委内瑞拉，尽管这两种疾病的几个地方性疫源地重合，但尚无哺乳动物同时感染的报告。对从科赫德斯州、阿拉瓜州、安索阿特吉州、瓜里科州、米兰达州和首都区采集的527份来自多种哺乳动物（家犬、驴、负鼠、骡、褐家鼠、马、小食果蝠、家猫、野猪、牛、山羊和格兰纳达松鼠）的滤纸血样进行了克氏锥虫-利什曼原虫属混合感染的分子诊断。通过对动基体小环DNA（kDNA）和卫星DNA（sDNA）进行PCR扩增来确定克氏锥虫感染情况。利什曼原虫属感染通过利什曼原虫巢式PCR（Ln-PCR）和核糖体DNA内转录间隔区1 PCR（ITS1-PCR）检测。克氏锥虫感染率为23.5%，利什曼原虫属感染率为12.9%，混合感染率为5.7%。负鼠是每种寄生虫病（克氏锥虫34.3%，利什曼原虫属20.0%）和混合感染（14.3%）感染率最高的物种。安索阿特吉州是每种寄生虫病（克氏锥虫64.9%，利什曼原虫属64.9%）和混合感染（43.2%）感染率最高的州。在未被报告为克氏锥虫（驴和骡）和利什曼原虫属（骡和野猪）天然宿主的物种中也检测到了感染。在评估的几个共同流行地区，混合感染在哺乳动物中是一种常见现象。
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