Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, Hunan 410013, China.
ACS Appl Mater Interfaces. 2022 Jan 26;14(3):3752-3761. doi: 10.1021/acsami.1c20170. Epub 2022 Jan 11.
Sensitive, rapid, and low-cost detection of drug traces in sewage is very important for drug monitoring and control. In this study, a dual functional and recyclable magnetic fluorescent molecularly imprinted polymers (MFMIPs) sensor with high sensitivity for rapid detection and purification of methcathinone in sewage was developed. MFMIPs was prepared via molecular imprinting and conjugation with carbon dots as a fluorescent reporter on FeO (FeO-MIPs@CDs). With strong recognition and adsorption toward methcathinone by the specific cavities on the surface of MFMIPs, the fluorescence of the sensor could dramatically be quenched once anchored with methcathinone. Under optimal conditions, the MFMIPs sensor presented high sensitivity with a linear range of 0.5-100 nM and a detection limit of 0.2 nM, which would be used to monitor drug prevalence and consumption within a certain region. This sensor was applied to the assay of methcathinone in sewage samples collected from Yuebeiyuan, Yanghu, and Xujiahu sewage pumping stations of Yuelu District. The calculated concentrations of methcathinone were 4.80, 15.33, and 8.59 nM in sewage samples, which were in good agreement with data tested by LC-MS/MS. For another function, MFMIPs exhibited purification toward methcathinone and the adsorption capacity was about 0.27 mg/g in a real sewage sample. Moreover, the sensor could be recycled and reused at least five times with the aid of an external magnetic field. Collectively, with good analytical performance and excellent recognition and selectivity to methcathinone, the proposed sensing system based on the magnetic core and molecularly imprinted polymers would open a door to establish highly sensitive and effective sensing systems for sewage analysis and purification.
灵敏、快速且低成本地检测污水中的药物痕迹对于药物监测和控制非常重要。在这项研究中，开发了一种具有高灵敏度的双重功能和可回收磁性荧光分子印迹聚合物（MFMIPs）传感器，用于快速检测和净化污水中的甲卡西酮。MFMIPs 通过分子印迹和与碳点（FeO-MIPs@CDs）共轭制备而成，作为荧光报告物。通过 MFMIPs 表面的特定腔体能强烈识别和吸附甲卡西酮，一旦与甲卡西酮结合，传感器的荧光就会显著猝灭。在最佳条件下，MFMIPs 传感器具有高灵敏度，线性范围为 0.5-100 nM，检测限为 0.2 nM，可用于监测特定区域内的药物流行和消耗情况。该传感器用于测定岳麓区月贝花园、杨湖和徐家湖污水泵站采集的污水样品中的甲卡西酮。计算出的甲卡西酮浓度分别为 4.80、15.33 和 8.59 nM，与 LC-MS/MS 测试的数据吻合良好。此外，MFMIPs 还具有净化甲卡西酮的功能，在实际污水样品中的吸附容量约为 0.27 mg/g。此外，借助外部磁场，该传感器至少可以重复使用五次。总之，基于磁核和分子印迹聚合物的传感系统具有良好的分析性能以及对甲卡西酮的出色识别和选择性，为建立用于污水分析和净化的高灵敏度和有效的传感系统开辟了道路。
