State Key Laboratory of Agricultural Microbiology, College of Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Division of Chemistry and Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore.
ACS Appl Bio Mater. 2021 Mar 15;4(3):2009-2019. doi: 10.1021/acsabm.0c00263. Epub 2020 Apr 22.
Metal-respiring bacteria are frequently used to recycle metal resources by biosynthesizing nanoparticles on its surface in environment treatment. However, further utilization of biogenetic nanoparticles through combining the advantages of both bacteria and nanoparticles is still limited. Herein, biogenetic Au@Ag nanoislands are utilized as the surface-enhanced Raman spectroscopy (SERS) substrate for quantitative detection. Specifically, Au@Ag nanoislands enhance the Raman signal surface plasmon resonance, while biomolecules (phospholipid, tyrosine, and phenylalanine, etc.) on bacterium serve as an internal standard to eliminate the discrepancy of the target SERS intensity in different hot spots. Gene-controlled biomolecules in bacteria guarantee the reproducibility of this SERS substrate. The generality of this analytical method is demonstrated by determining rhodamine 6G, malachite green, and uric acid. This discovery solves a pervasive problem in SERS analysis through a simple biogenetic nanosystem, which opens up an avenue to address scientific challenges by using versatile organisms from nature.
金属呼吸细菌通常用于在环境处理中通过在其表面生物合成纳米粒子来回收金属资源。然而，通过结合细菌和纳米粒子的优势进一步利用生物遗传纳米粒子的应用仍然有限。在此，生物遗传 Au@Ag 纳米岛被用作表面增强拉曼光谱 (SERS) 基底用于定量检测。具体来说，Au@Ag 纳米岛通过表面等离子体共振增强拉曼信号，而细菌上的生物分子（磷脂、酪氨酸和苯丙氨酸等）则作为内标消除不同热点中目标 SERS 强度的差异。细菌中的基因控制生物分子保证了这个 SERS 基底的重现性。该分析方法的通用性通过测定罗丹明 6G、孔雀石绿和尿酸得到了证明。这项发现通过一个简单的生物遗传纳米系统解决了 SERS 分析中的一个普遍问题，为利用自然界中多用途的生物来解决科学挑战开辟了道路。
