Forschungszentrum Jülich GmbH Jülich Centre for Neutron Science (JCNS-1) and Institute for Complex Systems (ICS-1), Wilhelm-Johnen-Straße, Jülich 52428, Germany.
Leibniz Institute of Polymer Research Dresden e. V., Hohe Straße 6, Dresden 01069, Germany.
ACS Appl Bio Mater. 2021 Feb 15;4(2):1720-1730. doi: 10.1021/acsabm.0c01495. Epub 2021 Jan 26.
This paper reports an approach for the fabrication of shape-changing bilayered scaffolds, which allow the growth of aligned skeletal muscle cells, using a combination of 3D printing of hyaluronic acid hydrogel, melt electrowriting of thermoplastic polycaprolactone-polyurethane elastomer, and shape transformation. The combination of the selected materials and fabrication methods allows a number of important advantages such as biocompatibility, biodegradability, and suitable mechanical properties (elasticity and softness of the fibers) similar to those of important components of extracellular matrix (ECM), which allow proper cell alignment and shape transformation. Myoblasts demonstrate excellent viability on the surface of the shape-changing bilayer, where they occupy space between fibers and align along them, allowing efficient cell patterning inside folded structures. The bilayer scaffold is able to undergo a controlled shape transformation and form multilayer scroll-like structures with cells encapsulated inside. Overall, the importance of this approach is the fabrication of tubular constructs with a patterned interior that can support the proliferation and alignment of muscle cells for muscle tissue regeneration.
本文报道了一种制造形状变化双层支架的方法，该方法结合了透明质酸水凝胶的 3D 打印、热塑性聚己内酯-聚氨酯弹性体的熔融静电纺丝和形状转变，允许排列整齐的骨骼肌细胞生长。所选材料和制造方法的结合具有许多重要的优点，如生物相容性、可生物降解性和合适的机械性能（纤维的弹性和柔软性）类似于细胞外基质（ECM）的重要组成部分，这允许适当的细胞排列和形状转变。成肌细胞在形状变化双层的表面表现出极好的活力，它们占据纤维之间的空间并沿纤维排列，允许在折叠结构内部进行有效的细胞图案化。双层支架能够进行受控的形状转变，并形成具有内部图案的多层螺旋状结构，内部包裹着细胞。总的来说，这种方法的重要性在于制造具有图案化内部的管状结构，这些结构可以支持肌肉细胞的增殖和排列，用于肌肉组织再生。
