Department of Gastroenterological Surgery, Nagoya City University Graduate School of Medical Sciences and Medical School, Mizuho‑cho, Mizuho‑ku, Nagoya, Aichi 467-8601, Japan.
Department of Gastroenterological Surgery, Nagoya City University West Medical Center, Kita‑ku, Nagoya, Aichi 462-8508, Japan.
Oncol Rep. 2022 Mar;47(3). doi: 10.3892/or.2022.8259. Epub 2022 Jan 11.
10Z‑Hymenialdisine is a natural product derived from the marine sponge . 10Z‑Hymenialdisine has anti‑inflammatory effects exerted through NF‑κB; however, it is unclear whether 10Z‑Hymenialdisine has anti‑angiogenic effects in cancer cells. In the present study, both the anti‑angiogenic and antimetastatic effects of this compound in pancreatic cancer were investigated. It was initially confirmed that 10Z‑Hymenialdisine significantly inhibited the proliferation of pancreatic cancer cells. Next, using both reverse transcription‑quantitative PCR and ELISA, it was demonstrated that 10Z‑Hymenialdisine significantly suppressed the expression of VEGF and IL‑8 mRNAs and proteins in pancreatic cancer. Immunohistochemical analysis revealed that 10Z‑Hymenialdisine inhibited NF‑κB activity in pancreatic cancer cell lines. It was also identified that 10Z‑Hymenialdisine inhibited tube formation in EA.hy926 cells. , 10Z‑Hymenialdisine significantly inhibited the growth of BxPC‑3 pancreatic cancer cells that were subcutaneously injected into model mice. In conclusion, the present study demonstrated that 10Z‑Hymenialdisine exerted anti‑angiogenic effects by suppressing NF‑κB activity and angiogenic factors, such as VEGF and IL‑8, in pancreatic cancer cell lines. 10Z‑Hymenialdisine has potential applications as a novel therapeutic agent for the treatment of pancreatic cancer.
10Z-海鞘素是一种天然产物，来源于海洋海绵。10Z-海鞘素具有通过 NF-κB 发挥抗炎作用的特性；然而，其是否在癌细胞中具有抗血管生成作用尚不清楚。在本研究中，研究了该化合物在胰腺癌中的抗血管生成和抗转移作用。首先证实 10Z-海鞘素可显著抑制胰腺癌细胞的增殖。接下来，通过逆转录定量 PCR 和 ELISA 实验，证实 10Z-海鞘素可显著抑制胰腺癌中 VEGF 和 IL-8 mRNA 和蛋白的表达。免疫组织化学分析显示 10Z-海鞘素抑制了胰腺癌细胞系中 NF-κB 的活性。此外，还发现 10Z-海鞘素抑制了 EA.hy926 细胞的管形成。10Z-海鞘素显著抑制了皮下注射到模型小鼠中的 BxPC-3 胰腺癌细胞的生长。综上所述，本研究表明 10Z-海鞘素通过抑制 NF-κB 活性和血管生成因子（如 VEGF 和 IL-8）在胰腺癌细胞系中发挥抗血管生成作用。10Z-海鞘素具有作为治疗胰腺癌的新型治疗剂的应用潜力。
