Chen Hui-Jiuan, Lin Di-An, Liu Fanmao, Zhou Lingfei, Liu Di, Lin Zhihong, Yang Chengduan, Jin Quanchang, Hang Tian, He Gen, Xie Xi
The First Affiliated Hospital of Sun Yat-Sen University, State Key Laboratory of Optoelectronic Materials and Technologies, School of Electronics and Information Technology, Sun Yat-Sen University, Guangzhou, China.
Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637, United States.
ACS Appl Bio Mater. 2018 Aug 20;1(2):374-381. doi: 10.1021/acsabm.8b00102. Epub 2018 Jul 20.
Bioactive functional probiotics play an important role in many health applications such as maintaining skin health and the immunity of the human host. Artificial supplementation of probiotics would enhance immune functions as well as regulate skin health. However, simple and effective methods to deliver probiotics into the dermis to regulate local dermal tissue are still lacking. Furthermore, microneedles have been used for transdermal drug delivery in a pain-free manner, yet there were few reported methods to deliver living microbes via microneedles. In this work, we developed a technique to deliver bioactive functional probiotics, using lactobacillus as the model probiotic, into local dermis by dissolvable microneedles. The transdermal delivery of probiotics might enhance local skin regulation and immunity, and dissolvable microneedles served as a safe and pain-free tool for dermal microbial delivery. Lactobacillus was encapsulated in dissolvable microneedles with high viability by a centrifugation casting method. The microneedles rapidly dissolved after skin penetration, releasing the lactobacillus into the subcutaneous space, without causing local tissue irritation. The lactobacillus was functionally bioactive following transdermal delivery, actively synthesizing lactic acid both ex vivo and in vivo. Our technique provided a safe, effective, and convenient approach for the transdermal delivery of probiotics into local skin, with the potential to improve skin health and immunity.
生物活性功能益生菌在许多健康应用中发挥着重要作用，如维持皮肤健康和人类宿主的免疫力。人工补充益生菌可增强免疫功能并调节皮肤健康。然而，目前仍缺乏将益生菌输送到真皮中以调节局部皮肤组织的简单有效方法。此外，微针已被用于无痛的经皮给药，但通过微针输送活微生物的报道方法很少。在这项工作中，我们开发了一种技术，以乳酸杆菌作为模型益生菌，通过可溶解微针将生物活性功能益生菌输送到局部真皮中。益生菌的经皮输送可能会增强局部皮肤调节和免疫力，可溶解微针作为一种安全无痛的皮肤微生物输送工具。通过离心浇铸法，乳酸杆菌以高活力封装在可溶解微针中。微针在穿透皮肤后迅速溶解，将乳酸杆菌释放到皮下空间，而不会引起局部组织刺激。经皮输送后，乳酸杆菌具有功能生物活性，在体外和体内都能积极合成乳酸。我们的技术为将益生菌经皮输送到局部皮肤提供了一种安全、有效且方便的方法，具有改善皮肤健康和免疫力的潜力。
