Kondibaeva Zhanat B, Yespembetov Bolat A, Abeuov Khairulla B, Mussayeva Assiya K, Siyabekov Sarsenbek T, Nussupova Saltanat T, Akmatova Elmira K, Pazylov Yerlan K, Maikhin Kydyrbay T, Syrym Nazym S
Laboratory of Diagnostic of the Infectious Diseases , Research Institute for Biological Safety Problems, Gwardeiski, Kazakhstan.
Laboratory of Microbiology , Research Institute for Biological Safety Problems, Gwardeiski, Kazakhstan.
Vet World. 2021 Nov;14(11):2957-2963. doi: 10.14202/vetworld.2021.2957-2963. Epub 2021 Nov 24.
The Aujeszky's disease, also known as Pseudorabies, remains one of the most problematic fulminant diseases in domestic animals, affecting the central nervous system. The study aimed to investigate the effect of an inactivated vaccine against Aujeszky's disease based on "Kordai" virus strain.
To test the inactivation of the "Kordai" strain (grown by the roller method in VNK-21/13 cell culture with an infectious titer of at least 7.5 lg TCD/ml) which is causative of Aujeszky's disease, next-generation teotropin and propolis preparations were usedin concentrations of 0.1%, 0.08%, and 0.04%.
As a result of comparative studies on the optimization of parameters for inactivating the "Kordai" virus strain, it was established that teotropin is a more effective inactivant than propolis. At the same time, the optimal final concentration of teotropin for inactivation was 0.1%, along with a reaction medium temperature of 37°C, pH of 7.4-7.6, and duration of inactivation of 14 h. The titer of virus-neutralizing activity (VNA) of antibodies at the pH (neutralization reactions) in vaccinated sheep of 10-12 months of age was 7.5±0.3, Ig TCID/ml (tissue culture infectious dose 50%), and 3.5±0.3 in the cell culture VNK-21/13 (culture of Syrian hamster kidney cells).
To determine colostral immunity in newborn lambs, the method of metabolic status correction was used to vaccinate lambs obtained from immune sheep 4 months after birth. The results showed that lambs obtained from immune sheep had high VNA titers. A sustained immune response in vaccinated animals was obtained after double vaccination.
奥耶斯基氏病，又称伪狂犬病，仍是家畜中最具问题的暴发性疾病之一，会影响中枢神经系统。本研究旨在调查基于“科尔代”病毒株的奥耶斯基氏病灭活疫苗的效果。
为检测导致奥耶斯基氏病的“科尔代”毒株（通过滚瓶法在VNK - 21/13细胞培养物中培养，感染滴度至少为7.5 lg TCD/ml）的灭活情况，使用了浓度为0.1%、0.08%和0.04%的下一代泰乐菌素和蜂胶制剂。
通过对“科尔代”病毒株灭活参数优化的比较研究，确定泰乐菌素比蜂胶是更有效的灭活剂。同时，灭活的泰乐菌素最佳终浓度为0.1%，反应介质温度为37°C，pH为7.4 - 7.6，灭活持续时间为14小时。10 - 12月龄接种疫苗绵羊在pH值（中和反应）下抗体的病毒中和活性（VNA）滴度为7.5±0.3，Ig TCID/ml（组织培养感染剂量50%），在细胞培养物VNK - 21/13（叙利亚仓鼠肾细胞培养物）中为3.5±0.3。
为确定新生羔羊的初乳免疫力，采用代谢状态校正方法对出生后4个月从免疫绵羊获得的羔羊进行接种。结果表明，从免疫绵羊获得的羔羊具有高VNA滴度。在进行两次接种后，接种动物获得了持续的免疫反应。
