Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Genética Molecular de Microrganismos, Rio de Janeiro, RJ, Brasil.
Universidade de Vassouras, Faculdade de Ciências Médicas de Maricá, Maricá, RJ, Brasil.
Mem Inst Oswaldo Cruz. 2022 Jan 10;116:e210247. doi: 10.1590/0074-02760210247. eCollection 2022.
Mycolicibacterium fortuitum is an opportunistic pathogen associated with human and animal infection worldwide. Studies concerning this species are mainly represented by case reports, some of them addressing drug susceptibility with a focus on a specific geographic region, so there is a gap in relation to the global epidemiological scenario.
We aimed determine the global epidemiological scenario of M. fortuitum and analyse its traits associated with pathogenicity.
Based on publicly available genomes of M. fortuitum and a genome from Brazil (this study), we performed a genomic epidemiology analysis and in silico and in vitro characterisation of the resistome and virulome of this species.
Three main clusters were defined, one including isolates from the environment, human and animal infections recovered over nearly a century. An apparent intrinsic resistome comprises mechanisms associated with macrolides, beta-lactams, aminoglycosides and antitubercular drugs such as rifampin. Besides, the virulome presented Type VII secretion systems (T7SS), including ESX-1, ESX-3, ESX-4 and ESX-4-bis, some of which play a role on the virulence of Mycobacteriaceae species.
Here, M. fortuitum was revealed as a reservoir of an expressive intrinsic resistome, as well as a virulome that may contribute to its success as a global opportunist pathogen.
偶然分枝杆菌是一种机会性病原体，与全球范围内的人类和动物感染有关。关于该物种的研究主要以病例报告为主，其中一些报告涉及药物敏感性，重点关注特定地理区域，因此在全球流行病学方面存在差距。
我们旨在确定偶然分枝杆菌的全球流行病学情况，并分析其与致病性相关的特征。
基于公开的偶然分枝杆菌基因组和巴西的一个基因组（本研究），我们进行了基因组流行病学分析以及该物种的耐药组和毒力组的计算机模拟和体外分析。
确定了三个主要的聚类，其中一个聚类包括来自环境、人类和动物感染的分离株，这些分离株在近一个世纪中被回收。一个明显的固有耐药组包括与大环内酯类、β-内酰胺类、氨基糖苷类和利福平（抗结核药物）等药物相关的机制。此外，毒力组呈现出 VII 型分泌系统（T7SS），包括 ESX-1、ESX-3、ESX-4 和 ESX-4-bis，其中一些在分枝杆菌属物种的毒力中发挥作用。
偶然分枝杆菌是一个丰富的固有耐药组的储库，也是一个毒力组，这可能有助于它成为一种全球性的机会性病原体。
