Geng Huimin, Dai Qiong, Sun Haifeng, Zhuang Liping, Song Aixin, Caruso Frank, Hao Jingcheng, Cui Jiwei
Key Laboratory of Colloid and Interface Chemistry of the Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China.
ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
ACS Appl Bio Mater. 2020 Feb 17;3(2):1258-1266. doi: 10.1021/acsabm.9b01138. Epub 2020 Jan 23.
Injectable and sprayable hydrogels have attracted considerable attention for application in the biomedical field owing to their high moldability and efficiency in encapsulating therapeutics and cells. Herein, we report the spontaneous assembly of injectable and sprayable hydrogels via a one-step mixing of solutions of tannic acid (TA) and -carboxymethyl chitosan (CMCS) without an external stimulus. The presence of 1,4-benzenediboronic acid (BDBA) improves the mechanical properties and reduces the gelation time of the resulting hydrogels. The hydrogels assemble via hydrogen bonds between TA and CMCS as well as via dynamic boronate ester bonds between TA and BDBA, as confirmed by Fourier transform infrared spectroscopy. Balancing the interactions between the three components (CMCS/TA/BDBA) is essential for the construction of the hydrogels. The moduli of the CMCS-TA-BDBA hydrogels initially increase as the amount of BDBA increases and decrease after reaching a maximum value at a BDBA-to-TA molar ratio of 3:1. The CMCS-TA-BDBA hydrogels with interconnected porous morphologies display rapid gelation (∼10 s), biocompatibility, self-healing, injectable, and sprayable abilities. In addition, the hydrogels can be used for hemostasis. The extent of bleeding in mouse livers treated with the hydrogels could be reduced extensively from 240 (nontreated mouse livers) to 55 mg (77% reduction). The reported hydrogels coupled with the combination of functionality and biological activity make them promising hemostatic materials for biomedical applications.
可注射和可喷雾水凝胶因其在封装治疗剂和细胞方面的高成型性和效率，在生物医学领域的应用中引起了相当大的关注。在此，我们报道了通过单宁酸（TA）和羧甲基壳聚糖（CMCS）溶液的一步混合，在无外部刺激的情况下自发组装可注射和可喷雾水凝胶。1,4-苯二硼酸（BDBA）的存在改善了所得水凝胶的机械性能并缩短了凝胶化时间。傅里叶变换红外光谱证实，水凝胶通过TA与CMCS之间的氢键以及TA与BDBA之间的动态硼酸酯键组装而成。平衡三种组分（CMCS/TA/BDBA）之间的相互作用对于水凝胶的构建至关重要。CMCS-TA-BDBA水凝胶的模量最初随着BDBA含量的增加而增加，在BDBA与TA的摩尔比为3:1时达到最大值后下降。具有相互连接的多孔形态的CMCS-TA-BDBA水凝胶表现出快速凝胶化（约10秒）、生物相容性、自愈性、可注射和可喷雾能力。此外，水凝胶可用于止血。用水凝胶处理的小鼠肝脏的出血程度可从240（未处理的小鼠肝脏）大幅降低至55毫克（减少77%）。所报道的水凝胶结合了功能和生物活性，使其成为生物医学应用中有前景的止血材料。
