Li Yu, Zhao Lihua, Yao Yuan, Guo Xuefeng
Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R. China.
ACS Appl Bio Mater. 2020 Jan 21;3(1):68-85. doi: 10.1021/acsabm.9b00840. Epub 2019 Dec 31.
Single-molecule detection is a rapidly developing area within the analytical chemistry field that requires ultrasensitive technologies to detect a range of molecules. Over the past few decades, various optically-, mechanically-, and electrically-based strategies have been employed for single-molecule detection to uncover information in biological processes. These strategies enable real-time monitoring with single-molecule/single-event sensitivity. In addition, their high temporal resolution enables investigation of the underlying mechanisms of biological functions from static to dynamic, from qualitative to quantitative, and from one to multiple disciplines. In this review, we provide a brief overview of the prominent, real-time single-molecule detection nanotechnologies and their potential applications within the life science fields.
单分子检测是分析化学领域中一个快速发展的领域，它需要超灵敏技术来检测一系列分子。在过去几十年里，人们采用了各种基于光学、机械和电学的策略进行单分子检测，以揭示生物过程中的信息。这些策略能够以单分子/单事件灵敏度进行实时监测。此外，它们的高时间分辨率使得能够从静态到动态、从定性到定量、从单一学科到多学科研究生物功能的潜在机制。在这篇综述中，我们简要概述了杰出的实时单分子检测纳米技术及其在生命科学领域的潜在应用。
