Balavigneswaran Chelladurai Karthikeyan, Venkatesan Ramya, Karuppiah Prakash Shyam, Kumar Gaurav, Paliwal Pankaj, Krishnamurthy Sairam, Kadalmani Balamuthu, Mahto Sanjeev Kumar, Misra Nira
Tissue Engineering and Biomaterials Lab, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai-600036, Tamil Nadu, India.
Department of Animal Science, Bharathidasan University, Tiruchirappalli-620024, Tamil Nadu, India.
ACS Appl Bio Mater. 2020 Jan 21;3(1):197-207. doi: 10.1021/acsabm.9b00680. Epub 2019 Dec 20.
Earlier, we had reported the synthesis and characterization of star-shaped poly(d,l-lactide)--gelatin (ss-pLG) to improve cell adhesion and proliferation, but the stability of ss-pLG scaffolds remained a persistent issue. Here we show an increase in the stability of ss-pLG using 3-glycidoxypropyl trimethoxysilane (GPTMS) as a covalent cross-linker (h-ss-pLG). The rate of cell proliferation within Hep-G2 cultured h-ss-pLG scaffolds increased until the third day, and afterward it drastically declined. Further, we identified the release of inorganic silica from GPTMS cross-linked h-ss-pLG, which may be associated with the decrease in the rate of HepG2 cell proliferation. However, the cross-linking did not affect red blood cells (RBCs) and they were completely hemocompatible. In addition, our experiments in female rats showed that the hybrid h-ss-pLG scaffolds were not degraded completely after 4 weeks, as they were covalently cross-linked with silane. These results suggest the significance of the cross-linker selection, which is one of the other key factors, and needs to be considered while designing scaffolds.
此前，我们曾报道过星形聚（d，l-丙交酯）-明胶（ss-pLG）的合成与表征，以改善细胞黏附和增殖，但ss-pLG支架的稳定性仍是一个长期存在的问题。在此，我们展示了使用3-缩水甘油氧基丙基三甲氧基硅烷（GPTMS）作为共价交联剂（h-ss-pLG）来提高ss-pLG的稳定性。在Hep-G2培养的h-ss-pLG支架内，细胞增殖速率在第三天之前增加，之后急剧下降。此外，我们确定了GPTMS交联的h-ss-pLG中无机硅的释放，这可能与HepG2细胞增殖速率的降低有关。然而，交联并不影响红细胞（RBC），它们具有完全的血液相容性。此外，我们在雌性大鼠身上的实验表明，由于与硅烷共价交联，混合的h-ss-pLG支架在4周后并未完全降解。这些结果表明了交联剂选择的重要性，交联剂选择是另一个关键因素之一，在设计支架时需要加以考虑。
