Lv Xiaoyi, Xu Xiaoyun, Miao Tian, Zang Xufeng, Geng Chao, Li Yanping, Cui Bo, Fang Yishan
State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
College of Science, Huzhou University, Huzhou, Zhejiang 313000, China.
ACS Appl Bio Mater. 2020 Dec 21;3(12):8933-8942. doi: 10.1021/acsabm.0c01201. Epub 2020 Dec 3.
9,10-Diphenylanthracene cubic nanoparticles (DPA CNPs) with aggregation-induced emission characteristic (AIEgens) were synthesized through a facile reprecipitation method; then, a bright and stable electrochemiluminescence (ECL) signal can be observed when the DPA CNPs were modified at the glassy carbon electrode (GCE) in the presence of Tri--propylamine (TPA). This phenomenon is ascribed to the molecules with restricted movement that greatly blocked the energy leakage during the relaxation of the excited state, which facilitated the emission of energy in the form of photons. In addition, the size confinement effect of DPA CNPs in the aggregated state effectively enhanced the ECL emission. The application of DPA CNPs with AIE characteristics in an electrochemiluminescence immunosensor has not been reported. In this contribution, a free-label aggregation-induced electrochemiluminescence immunosensor based on DPA CNPs was fabricated and a simple strategy for ultrasensitive detection of aflatoxin B (AFB) was proposed. The ECL signal is quenched linearly in the range of 0.01 pg/mL to 100 ng/mL for AFB, and the detection limit is 3 fg/mL. In summary, the prepared sensor exhibits high sensitivity, acceptable accuracy, good anti-interference ability and stability, and satisfactory detection toward AFB in walnut samples. Therefore, the fabricated immunosensor will have significant applications in the fields of food, medicine, and so on.
通过简便的再沉淀法合成了具有聚集诱导发光特性（聚集诱导发光体）的9,10-二苯基蒽立方纳米颗粒（DPA CNPs）；然后，当DPA CNPs在三丙胺（TPA）存在下修饰于玻碳电极（GCE）时，可观察到明亮且稳定的电化学发光（ECL）信号。这种现象归因于分子运动受限，极大地阻碍了激发态弛豫过程中的能量泄漏，从而促进了以光子形式的能量发射。此外，聚集态DPA CNPs的尺寸限制效应有效地增强了ECL发射。具有AIE特性的DPA CNPs在电化学发光免疫传感器中的应用尚未见报道。在本研究中，制备了基于DPA CNPs的无标记聚集诱导电化学发光免疫传感器，并提出了一种超灵敏检测黄曲霉毒素B（AFB） 的简单策略。AFB的ECL信号在0.01 pg/mL至100 ng/mL范围内呈线性猝灭，检测限为3 fg/mL。综上所述，所制备的传感器具有高灵敏度、可接受的准确度、良好的抗干扰能力和稳定性，对核桃样品中的AFB检测效果令人满意。因此，所制备的免疫传感器在食品、医药等领域将具有重要应用。
